固相萃取-高效液相色谱法测定烟草样品中主要的植物多酚

 建立了先用体积分数为 80 %的甲醇水溶液回流提取烟草样品中的植物多酚 ,再采用Sep Pak C18固相微萃取小柱预分离脱脂的前处理方法 ,并选择了合适的分析烟草样品中植物多酚的高效液相色谱条件。采用C18柱 ,甲醇 磷酸二氢钾缓冲液为流动相进行梯度洗脱 ,烟草中主要的植物多酚均达到基线分离。提取各组分在其最大波长下的色谱图 ,根据其峰面积定量 ,并用紫外光谱图对烟草中主要的植物多酚进行辅助定性。在该方法中 ,多酚的标准回收率为 94%～ 10 5 %,RSD为 1 2 8%～ 1 49%。该方法可用于烟草样品中的植物多酚的测定。

Determination of Polyphenols in Tobacco by Solid PhaseExtraction and High Performance Liquid Chromatography

A high performance liquid chromatographic (HPLC) method for the determination of polyphenols in tobacco was studied. Polyphenols can be extracted from tobacco sample by refluxing with 80% (volume fraction) methanol, then subjected to degrease by solid phase extraction with Sep-Pak-C18 column. Chlorogenic acid, rutin, scopoletin, caffeic acid, scopolin and other polyphenols can be separated on a Nova-Pak-C18 column (3.9 mm i.d. x 150 mm) gradiently eluted with methanol and 0.05 mol.L-1 potassium dihydrogen phosphate buffer solution as mobile phase at a flow-rate of 0.5 mL.min-1. Polyphenols were determined at the maximum wavelength of each polyphenol, for chlorogenic acid 326.1 nm, rutin 354.8 nm, scopoletin 344.0 nm, caffeic acid 323.7 nm and scopolin 365.2 nm by photodiode array detector. The main polyphenols in tobacco can be identified with UV spectroscopy. The recoveries of tobacco polyphenols were 94%-105%, and the relative standard deviations were 1.28%-1.49%. This method can be applied to the determination of polyphenols in tobacco with satisfactory results.