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猪毛水解母液中氨基酸的反相高效液相色谱分析
引用本文:金燕,沈恩云,金鑫荣,孙科夫.猪毛水解母液中氨基酸的反相高效液相色谱分析[J].色谱,1995,13(3):220-222.
作者姓名:金燕  沈恩云  金鑫荣  孙科夫
作者单位:华东理工大学理学院
摘    要: A new method for separation and quantification of amino acids by pre-column derivatization withphenylisothiocyanate(PITC ) and reversed-phase HPLC is described. Seventeen amino acids were separatedin 30 min at room temperature.The method has been successfully applied to the determination of amino acidcomposition in the solution which was prepared by hydrolyzing pig hair and extration of cystine.

关 键 词:反相高效液相色谱  氨基酸  柱前衍生  猪毛水解母液

Sensitive Determination of Amino Acid Composition of MotherLiquor Prepared by Hydrolyzing PigHair with Reversed-Phase High Performance Liquid Chromatography(HPLC)
Jin Yan, Shen Enyun, Jin Xinrong and Sun Kefu.Sensitive Determination of Amino Acid Composition of MotherLiquor Prepared by Hydrolyzing PigHair with Reversed-Phase High Performance Liquid Chromatography(HPLC)[J].Chinese Journal of Chromatography,1995,13(3):220-222.
Authors:Jin Yan  Shen Enyun  Jin Xinrong and Sun Kefu
Abstract:new method for separation and quantification of amino acids by pre-column derivatization withphenylisothiocyanate(PITC ) and reversed-phase HPLC is described. Seventeen amino acids were separatedin 30 min at room temperature.The method has been successfully applied to the determination of amino acidcomposition in the solution which was prepared by hydrolyzing pig hair and extration of cystine.
Keywords:high performance liquid chromatography  amino acid  pre-column derivatization  mother liquor prepared by hydrolyzing pig hair  
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