痕量氨基糖和中性单糖的高效毛细管电泳及液相色谱分析

由于糖缀合物具有较弱的紫外吸收，采用了一种与单糖有高反应活性的新型紫外、荧光衍生试剂芴甲氧羰基肼（ＦＭＯＣ－Ｈｙｄｒａｚｉｎｅ）和芴甲氧羰基氯（ＦＭＯＣ－ｃｌ）对单糖进行衍生。利用高效毛细管电泳（ＨＰｃＥ）和液相色谱技术对衍生产物进行了分析。结果表明：以上衍生试剂可用于ｆｍｏｌ级单糖的测定。对单糖的ＨＰＣＥ分离因素进行了详细讨论。

Trace-Analysis of Amino Sugars and NeutralSaccharides by High Performance Capillary Electrophoresis(HPCE)and High Performance Liquid Chromatography(HPLC)

ecause of the low UV absorbance of carbohydrates, the active fluorescence and ultraviolet labellingreagents,9-fluorenylmethoxycarbonyl(FMOC ) hydrazine and 9-fluorenylmethoxycarbonyl chloride,havebeen utilized as derivatization reagents for monosaccharides in the present work.HPCE and HPLC coupledwith ultraviolet and fluorescence detector was studied as a means of separation and determination of sugars.The results show that monosaccharides derivatized with FMOC-hydrazine and FMOC-Cl can be determinedwith high sensitivity at fmol level. The HPCE separation conditions of monosaccharides were studied in de-tails.