毛细管区带电泳法测定卷烟中的生物碱

在磷酸缓冲体系中采用毛细管区带电泳法测定卷烟中的生物碱时,检测灵敏度低,分离度差。考察了卷烟中生物碱的 提取条件，分离缓冲溶液的类型、pH值和浓度，卷烟中生物碱测定方法的线性范围、检出限、重现性和回收率。结果发 现，当采用410 mmol/L的酒石酸溶液（pH 2.8）为缓冲体系时,卷烟中生物碱的检测灵敏度和分离度均有明显改善,烟碱 的线性范围为0.06~0.80 mg/L（其他生物碱为0.006~0.10 mg/L）,检出限为0.002~0.01 mg/L,相对标准偏差为2.2%~10%,回收率为87.6%~102%。

Determination of Alkaloids in Cigarettes by Capillary Zone Electrophoresis

The separation of cigarette alkaloids was reported and performed with tartaric acid buffer solution by capillary zone electrophoresis(CZE).A buffer solution of pH 2.8,410 mmol/L tartaric acid was used as the running buffer for the determination of cigarette alkaloids in CZE.The detection sensitivity and resolution with the tartaric acid buffer solution were better than those with phosphate buffer.The sample extraction conditions,buffer electrolyte,pH and concentrations were investigated for the alkaloids separation and determination.The linear range,detection limits,reproducibilities and recoveries of the cigarette alkaloids were 0.06-0.80 mg/L for nicotine(0.006-0.10 mg/L for other alkaloids),0.002-0.01 mg/L,2.2%-10% and 87.6%-102%,respectively.