| 糖蛋白结构质谱解析的样品前处理 |
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| 引用本文： | 周玮,刘晓慧,周新文,申华莉,杨芃原.糖蛋白结构质谱解析的样品前处理[J].色谱,2007,25(5):623-627. |
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| 作者姓名： | 周玮 刘晓慧 周新文 申华莉 杨芃原 |
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| 作者单位： | Institutes of Biomedical Sciences, Fudan University, Shanghai 200032,
China |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 基于高分辨率、高精度、高灵敏度及良好表现力的多级质谱,以标准糖蛋白辣根过
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| 关 键 词： | 糖蛋白结构 条件优化 样品前处理 质谱解析 |
| 文章编号： | 1000-8713（2007）05-0623-05 |
| 收稿时间： | 2007-8-14 |
| 修稿时间： | 2007-05-16 |
Sample Pre-Processing for Mass Spectrometric Analysis of Glycoprotein
Structure |
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| ZHOU Wei,LIU Xiaohui,ZHOU Xinwen,SHEN Huali,YANG Pengyuan.Sample Pre-Processing for Mass Spectrometric Analysis of Glycoprotein
Structure[J].Chinese Journal of Chromatography,2007,25(5):623-627. |
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| Authors: | ZHOU Wei LIU Xiaohui ZHOU Xinwen SHEN Huali YANG Pengyuan |
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| Institution: | Institutes of Biomedical Sciences, Fudan University, Shanghai 200032,
China |
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| Abstract: | A novel,simple and fast method based on a quadrupole ion trap(QIT) and a time-of-flight mass spectrograph was developed for the glycosylation site and glycan structure analysis of glycoprotein by in-solution digestion and in-gel digestion which did not need to enrich and label the glycopeptide.Horseradish peroxidase(HRP) and RNase B were utilised to optimize the conditions of sample pre-processing.The effects of the conditions were investigated separately and the optimal conditions were:heat denaturation of RNase B,chemical denaturation of HRP,RNase B digestion with endoproteinase Lys-C,HRP digestion with trypsin,12-16 h of digestion,50% acetonitrile-5% trifluoroacetic acid of the extraction solution,sandwich spotting method of the sample. |
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| Keywords: | glycoprotein structure mass spectrometric analysis sample pre-preparation conditions of optimization |
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