高效液相色谱法检测多种食品基体中残留的喹氧灵

建立了采用液相色谱检测大豆、花椰菜、樱桃、木耳、葡萄酒、茶叶、蜂蜜、猪肝、鸡肉、鳗鱼等多种食品基体中喹氧灵残留的方法。利用乙酸乙酯提取样品中残留的喹氧灵,用氨基固相萃取小柱净化;对于脂肪含量较高的样品,在进行固相萃取前采用凝胶渗透色谱净化技术去脂。方法的准确度与精密度较好,在添加浓度为0.010～5.0 mg/kg时,平均回收率及相对标准偏差分别为82%～96%及3.2%～11.8%;在0.050～50.0 mg/L范围内有良好的线性关系,检测限达0.010 mg/kg。该方法适用性广,能消除复杂基质带来的干扰,可用于各类食品中喹氧灵残留的分析。

Determination of quinoxyfen residue in foodstuffs of plant and animal origins by high performance liquid chromatography

A high performance liquid chromatographic (HPLC) method has been developed for the determination of quinoxyfen residue in various food matrixes including soybean, cauliflower, cherry, mushroom, wine, tea, honey, pork liver, chicken and eel. The analyte was extracted by ethyl acetate, and then purified with aminopropyl solid phase extraction (NH2 SPE) cartridge. Post-extraction gel permeation chromatography (GPC) was used for animal (except honey) and fishery products prior to NH2 SPE cleanup.The average recoveries and relative standard deviations (RSDs) for the analysis of all samples fortified in the range of 0.010-5.0 mg/kg were in the ranges of 82%-96% and 3.2%-11.8%, respectively. Good linearity was obtained in the concentration range from 0.050 to 50.0 mg/L. The limit of detection was 0.010 mg/kg. The proposed method was successfully applied to the analysis of quinoxyfen residue in various food samples.