丹参中3种丹参酮的超临界二氧化碳萃取及液相分析

 用超临界CO2 流体及共溶剂乙醇萃取丹参中的 3种丹参酮 ,分别采用正交设计法和系统法考察了萃取中的主要影响因素 ;采用高效液相法 (HPLC) ,在甲醇 水 (体积比为 80∶2 0 )溶液为流动相和检测波长为 2 80nm的条件下 ,以外标法检测了萃取产物中 3种丹参酮的含量。实验得到的最佳条件为 :萃取压力 2 0MPa ;萃取温度 4 5℃ ;分离温度 35℃ ;共溶剂 95 % (体积分数 )乙醇 ;流量 1 0mL/min。建立的HPLC测定方法简便快捷 ,准确度高 ,重现性良好 ,相关系数r为 0 9994～ 0 9998,相对标准偏差RSD为 2 37%～ 3 4 7%。

Extraction of Three Tanshinones from the Root of Salvia miltiorrhizaBunge by Supercritical Carbon Dioxide Fluid and Their Analysis with High Performance Liquid Chromatography

The extraction of three tanshinones from the root of Salvia miltiorrhiza Bunge by supercritical carbon dioxide (SC-CO2) fluid with ethanol as co-solvent was investigated using four-factor and three-level orthogonal design method and the systematic method. The optimized conditions were established as follows: 95% ethanol was selected as a co-solvent, flow rate of co-solvent was 1.0 mL/min, extraction pressure was 20 MPa, extraction temperature was 45 degrees C, separation temperature was 35 degrees C. The sample solution was separated on a Nova-Pak C18 column (3.9 mm i.d. x 300 mm, 5 microns) with CH3OH-H2O (80:20, V/V) as the mobile phase and detected at 280 nm. The correlation coefficients were 0.9994-0.9998 and the relative standard deviations were 2.37%-3.47%. The detection limits were 0.473 mg/L, 0.184 mg/L and 0.452 mg/L for tanshinone-II A, tanshinone-I and cryptotanshinone respectively.