柏子养心丸的毛细管电泳指纹图谱

建立了柏子养心丸(Baizi Yangxin Wan, BZYXW)毛细管区带电泳指纹图谱(CEFP)。采用三棱柱优化法优化背景电解质(BGE),以色谱指纹图谱分离量指数(RF)为实验条件优化的目标函数,最终确定BGE为50 mmol/L硼砂-50 mmol/L磷酸氢二钠-200 mmol/L硼酸-150 mmol/L碳酸氢钠(体积比为7:7:1:1,含4%乙腈,pH 9.70)。在其他优化的毛细管电泳条件为紫外检测波长228 nm,运行电压12 kV,重力进样25 s (高度14 cm)条件下,采用未涂层石英毛细管(70 cm(有效分离长度57 cm)×75 μm)分离,以阿魏酸峰为参照,确定了17个共有指纹峰。对样品聚类分析后用其中9批生成对照CEFP(RCEFP),以其为标准,用系统指纹定量法鉴别出12批BZYXW质量为: 3批好,1批良好,3批中,1批一般,4批劣。三棱柱优化法为BGE选择提供了重要参考,建立的BZYXW-CEFP精密度好、重现性高,可用于BZYXW的质量控制。

Capillary electrophoresis fingerprints of Baizi Yangxin Wan

The capillary electrophoresis fingerprint (CEFP) of Baizi Yangxin Wan (BZYXW) was established by capillary zone electrophoresis (CZE). The chromatographic fingerprint resolution index (RF) was applied to optimize the CEFP conditions. The electrophoretic separation was performed on a 75 cm (the effective length of 57 cm) x 75 microm uncoated fused silica capillary with 50 mmol/L sodium borate-50 mmol/L Na2HPO(4)-200 mmol/L H3BO(3)-150 mmol/L NaHCO3 (7:7:1:1, v/v/v/v) containing 4% acetonitrile (pH 9.70) as the background electrolyte (BGE), and the BGE was chosen by using the triangular prism optimization method. The running voltage was set at 12 kV, and the detection wavelength at 228 nm. The sample solution was injected into the capillary by hydraulic pressure in 25 s. The CEFPs were produced by the electropherograms from 12 batches of BZYXW, and the 17 co-possessing peaks were selected as the fingerprint peaks of BZYXW's CEFP by choosing ferulic acid peak as the referential peak. According to the results of classification, the reference CEFP (RCEFP) was synthesized from 12 batches of BZYXW. Taking the RCEFP for the qualified model, the systematically quantified fingerprint method(SQFM) was applied to evaluate the quality of 17 batches of BZYXW, in which the qualities of 3 batches was evaluated as good, 1 batch as fine, 3 batches as moderate, 1 batch as common, and 4 batches as inferior. The triangular prism optimization method was suggested to be good to optimize the BGE. The results showed that the BZYXW-CEFP was established with good precision and reproducibility, which can be served as a novel reference to identify and control the quality of BZYXW.