| Porous Monoliths: Stationary Phases of Choice for High Performance Liquid Chromatography in Various Formats |
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| 作者姓名： | SVEC Frantisek |
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| 作者单位： | Department of Chemistry, University of California, Berkeley, CA 94720-1460, USA |
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| 基金项目： | Supported by grants of the National Institute of General Medical Sciences, National Institutes of Health （GM-48354）, and the Materials Sciences and Engineering Division of the U. S. Department of Energy （DE-AC02-05CH11231）. |
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| 摘 要： | Modern porous monoliths have been conceived as a new class of stationary phases for high performance liquid chromatography (HPLC) in classical columns in the early 1990s and later extended to the capillary format. These monolithic materials are prepared using simple processes carried out in an external mold (inorganic monoliths) or within the confines of the column (organic monoliths and all capillary columns). These methods afford macroporous materials with large through-pores that enable applications in a rapid flow-through mode. Since all the mobile phase must flow through the monolith, the convection considerably accelerates mass transport within the monolithic separation medium and improves the separations. As a result, the monolithic columns perform well even at very high flow rates. The applications of monolithic capillary columns are demonstrated on numerous separations in the HPLC mode.
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| 关 键 词： | high performance liquid chromatography porous monoliths stationary phases synthetic polymers silica |
| 文章编号： | 1000-8713（2005）06-0585-10 |
| 收稿时间： | 2005-09-08 |
Porous Monoliths: Stationary Phases of Choice for High Performance Liquid Chromatography in Various Formats |
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| SVEC Frantisek.Porous Monoliths: Stationary Phases of Choice for High Performance Liquid Chromatography in Various Formats[J].Chinese Journal of Chromatography,2005,23(6):585-594. |
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| Authors: | SVEC Frantisek |
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| Institution: | Department of Chemistry, University of California, Berkeley, CA 94720-1460, USA |
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| Abstract: | Modern porous monoliths have been conceived as a new class of stationary phases for high performance liquid chromatography(HPLC) in classical columns in the early 1990s and later extended to the capillary format.These monolithic materials are prepared using simple processes carried out in an external mold(inorganic monoliths) or within the confines of the column(organic monoliths and all capillary columns).These methods afford macroporous materials with large through-pores that enable applications in a rapid flow-through mode.Since all the mobile phase must flow through the monolith,the convection considerably accelerates mass transport within the monolithic separation medium and improves the separations.As a result,the monolithic columns perform well even at very high flow rates.The applications of monolithic capillary columns are demonstrated on numerous separations in the HPLC mode. |
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| Keywords: | high performance liquid chromatography porous monoliths stationary phases synthetic polymers silica |
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