阳离子表面活性剂胶束电动毛细管色谱中的假峰现象

研究了以十六烷基三甲基溴化铵为准固定相的胶束电动毛细管色谱中假峰的起源。指出在一定条件下,一个组分可能出现两个峰。实验表明,被分析物双峰的相对峰面积取决于被分析物与表面活性剂反应的时间、温度以及表面活性剂在样品中的浓度。这意味着被分析物与表面活性剂之间的反应是一个慢过程,这种相互作用能够产生一种稳定的物质,并导致假峰的形成。根据实验结果,溶质与胶束之间的快速相互作用机理应被质疑。

Pseudo Peak Phenomena in Micellar Electrokinetic Capillary Chromatography by Using Cationic Surfactant

Using cetyltrimethylammonium bromide as pseudo stationary phase, the origin of pseudo peak in micellar electrokinetic capillary chromatography was investigated, relating to the fact that under certain conditions two peaks might come arise from a single component. Experiments further showed that the relative areas of the two peaks of an analyte were dependent on the reation time and temperature, under which reactions between analyte and surfactant occurred, and also dependent on concentration of surfactant in sample solutions. The results suggested that the interaction between analyte and surfactant is a slow process, and a stable substance might be formed via the interaction and lead to the formation of pseudo peaks. The fast interaction mechanism between the solute and the micelle could be queried according to the experimental results.