基于双定性原则的高效液相色谱-二极管阵列检测法分析中药组分

双定性是根据样品的保留时间和吸收光谱的特征峰进行复合定性的一种新方法。该文基于自行设计及组装的二极管阵列检测器，构建了一套液相色谱-二极管阵列检测系统。采用该色谱系统分别对6种中药饮片中的非法添加物金胺O和枣仁天麻胶囊中的有效成分五味子醇甲进行了分离及定性研究。结果显示，在蒲黄饮片和枣仁天麻胶囊样品色谱图中均存在与目标检测物保留时间接近的色谱峰，进一步通过吸收光谱的特征峰比对，均排除了目标物存在的可能。应用结果证明，基于保留时间/吸收光谱的双定性原则，可以有效排除样品中杂质的干扰，避免假阳性结果，为中药组分研究提供了参考方法。

Analysis of traditional Chinese medicine components by high performance liquid chromatography with diode array detection based on double qualitative principles

The double qualitative principle is a new composite qualitative method based on retention time and the characteristic peaks of the absorption spectra. Using a self-designed and assembled diode array detector (DAD), a high performance liquid chromatography (HPLC) system was constructed. The illegal additive auramine O in six kinds of herbal slices and the active ingredient schisandrin in Jujube kernel Tianma capsules were separated and qualitative analyzed using the HPLC-DAD system. The results showed that there were similar peaks in the chromatograms of pollen typhae and Jujube kernel Tianma capsules when comparing the target analytes. However, the probabilities of the targets were excluded by comparing the absorption spectra. The application results indicated that, based on the double qualitative principle of retention time/absorption spectrum, the interference of impurities in the samples could be well eliminated and the false positives could be avoided. This provides a reference method for the study of traditional Chinese medicine components.