钴酞菁功能化聚合物整体柱的制备及用于转铁蛋白糖肽的富集

建立了一种新型的聚合物整体柱制备方法，将四羧基钴酞菁（CoPcTc）引入聚（甲基丙烯酸缩水甘油酯-乙二醇二甲基丙烯酸酯）（Poly（GMA-EDMA））整体柱，用于转铁蛋白糖肽的富集。由于四羧基钴酞菁和糖肽之间的氢键和金属配位作用，制备的整体材料在用于糖肽富集时具有高效性和选择性。转铁蛋白经过功能化整体柱富集后，通过电喷雾四极杆飞行时间质谱分析，共捕获到17条糖肽。当转铁蛋白浓度降至8.8×10^-10mol/L时，仍然可以得到3条糖肽，说明合成的聚合物整体材料对微量蛋白样品分析具有很高的应用潜力。

Preparation of cobalt phthalocyanine functionalized polymer monolith and application to enrichment of transferrin glycopeptides

Poly(glycidyl methacrylate-ethyleneglycol dimethacrylate) (Poly(GMA-EDMA)) monolith functionalized with cobalt phthalocyanine tetracarboxylic acid (CoPcTc) was prepared. The monolith was used for transferrin (Tf) glycopeptide enrichment. By taking advantage of hydrogen bonds between isoindole subunits of phthalocyanine and glycans and coordination interaction between cobalt and glycopeptides, the monolithic material was efficient and selective. After enrichment of transferrin through the functionalized monolith, 17 glycopeptides were identified by electrospray ionization quadrupole time-of-flight mass spectrometry. When the concentration of transferrin was reduced to 8.8×10^-10mol/L, three glycopeptides could still be obtained. The present method has great potential for trace sample analysis.