| 反相高效液相色谱法制备松果菊苷标准品 |
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| 引用本文： | 雷厉,宋志宏,屠鹏飞,吴立军,陈发奎.反相高效液相色谱法制备松果菊苷标准品[J].色谱,2001,19(3):200-202. |
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| 作者姓名： | 雷厉 宋志宏 屠鹏飞 吴立军 陈发奎 |
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| 作者单位： | 1. 北京大学药学院天然药物学系,
2. 沈阳药科大学中药系, |
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| 基金项目： | 卫生部优秀青年科技人才专项科研基金(批准号:9822)和国家自然科学基金(批准号:30070887)资助 |
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| 摘 要： | 利用反相制备高效液相色谱结合溶剂萃取、大孔吸附树脂柱色谱和葡聚糖凝胶LH 2 0柱色谱方法 ,从管花肉苁蓉的乙醇提取物中纯化制备了苯乙醇苷类化合物松果菊苷的标准品 ,纯度达到 98%以上。方法操作简便 ,重现性好 ,可用于松果菊苷及其他苯乙醇苷类化合物的大量制备。
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| 关 键 词： | 反相制备高效液相色谱法 肉苁蓉 苯乙醇苷类 松果菊苷 |
| 文章编号： | 1000-8713(2001)03-0200-03 |
| 修稿时间： | 2000年9月18日 |
Separation of Echinacoside by Reversed-Phase Preparative High Performance Liquid Chromatography |
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| LEI Li ,SONG Zhi hong ,TU Peng fei ,WU Li jun ,CHEN Fa kui.Separation of Echinacoside by Reversed-Phase Preparative High Performance Liquid Chromatography[J].Chinese Journal of Chromatography,2001,19(3):200-202. |
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| Authors: | LEI Li SONG Zhi hong TU Peng fei WU Li jun CHEN Fa kui |
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| Institution: | Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University, Beijing 100083, China. |
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| Abstract: | Echinacoside, one kind of phenylethanoid glycosides (PhGs), was isolated from the stems of Cistanche tubulosa (Schenk) R. Wight with a series of steps, including solvent extraction, D101 polymer adsorption column separation, Sephadex LH-20 separation, C18 column reversed-phase preparative high performance liquid chromatographic (RP-prep-HPLC) preparation and polyamide thin-layer chromatographic detection. The purity of the product was over 98%. The chemical structure of echinacoside was identified by 1H NMR and 13C NMR. To find out the optimum condition of mobile phase of RP-prep-HPLC, several systems were used in this work. Finally acetonitrile-1% HCOOH water (18:82, V/V) system was found to be the best. On the other hand D101 polymer adsorption column and Sephadex LH-20 were also effective for PhGs separation. |
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| Keywords: | reversed phase preparative high performance liquid chromatography Herba Cistanches phenylethanoid glycoside echinacoside |
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