首页 | 本学科首页   官方微博 | 高级检索  
     检索      

高效液相色谱法测定全血中骨碱性磷酸酶的活力
引用本文:张咏梅,白秀珍,陈占文,张春先.高效液相色谱法测定全血中骨碱性磷酸酶的活力[J].色谱,2000,18(3):235-236.
作者姓名:张咏梅  白秀珍  陈占文  张春先
作者单位:1. 锦州医学院,辽宁,锦州,121001
2. 锦州妇幼保健站,辽宁,锦州,121000
3. 锦州工学院,辽宁,锦州,121000
摘    要: 建立了测定全血中骨碱性磷酸酶(BALP)的高效液相色谱法(HPLC)。色谱柱为SpectraPhysicsODS反相柱,以V(0.01mol/L乙酸,pH4.8)∶V(甲醇)=70∶30的溶液为流动相,流速为0.8mL/min,检测波长为275nm。当BALP的质量浓度为8~200mg/L时,其活性对峰面积呈良好的线性关系(r=0.996),检测限为3ng。该方法简便、快速、可靠,可用于临床测定全血中的BALP。

关 键 词:高效液相色谱法  骨碱性磷酸酶  全血

Determination of Activity of Bone Alkaline Phosphatase in Whole Serum by High Performance Liquid Chromatography
ZHANG Yong-mei,BAI Xiu-zhen,CHEN Zhan-wen,ZHANG Chun-xian.Determination of Activity of Bone Alkaline Phosphatase in Whole Serum by High Performance Liquid Chromatography[J].Chinese Journal of Chromatography,2000,18(3):235-236.
Authors:ZHANG Yong-mei  BAI Xiu-zhen  CHEN Zhan-wen  ZHANG Chun-xian
Institution:Jinzhou Medical College, Jinzhou 121001, China. zhangym6311@263.net
Abstract:An analytical method has been established for the determination of bone alkaline phosphatase (BALP) activity using high performance liquid chromatography(HPLC). The stainless steel column was 22 cm x 4.6 mm i.d. packed with totally porous, spherical silica particles. A solution of methanol and 0.01 mol/L CH3COOH(30/70, V/V) adjusted to pH 4.8 was employed as the mobile phase. The flow rate was 0.8 mL/min. Chromatography was performed with ultraviolet detector at 275 nm. Linear calibration curve for BALP was measured within the range of 8-200 mg/L with correlation coefficient of 0.996. The lowest detection limit was 3 ng. The HPLC method described is simple, rapid and reliable and suitable for clinical monitoring.
Keywords:high  performance  liquid  chromatography  bone  alkaline phosphatase  whole  blood
本文献已被 CNKI 维普 万方数据 等数据库收录!
点击此处可从《色谱》浏览原始摘要信息
点击此处可从《色谱》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号