Rapid Detection of AIB1 in Breast Cancer Cells Based on Aptamer-Functionalized Nanomotors |
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| Authors: | Dr Mara Beltrán-Gastélum Dr Berta Esteban-Fernández de Ávila Dr Hua Gong Dr Pooyath Lekshmy Venugopalan Prof Dr Tibor Hianik Prof Dr Joseph Wang Veronika Subjakova |
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| Institution: | 1. Department of Nanoengineering, University of California San Diego, La Jolla, California, 92093 United States;2. Department of Nuclear Physics and Biophysics Faculty of Mathematics, Physics and Informatics, Comenius University, Mlynská dolina F1, 842 48 Bratislava, Slovakia |
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| Abstract: | Herein, we report ultrasound-propelled graphene-oxide coated gold nanowire motors, functionalized with fluorescein-labeled DNA aptamers (FAM-AIB1-apt), for qualitative detection of overexpressed AIB1 oncoproteins in MCF-7 breast cancer cells. The movement of nanomotors under the ultrasound field facilitated intracellular uptake and resulted in a faster aptamer binding with the target protein and thus faster fluorescence recovery. The propulsion behavior of the aptamer functionalized nanomotors greatly enhanced the fluorescence intensity compared to static conditions. The new aptamer@nanomotor-based strategy offers considerable potential for further development of sensing methodologies towards diagnosis of breast cancer. |
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| Keywords: | aptamer AIB1 nanomotor fluorescence graphene |
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