ATR/FTIR characterization of Steinernema glaseri and Heterorhabditis indica |
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| Authors: | Ernesto San-Blas Mayamarú Guerra Edgar Portillo Iván Esteves Nestor Cubillán Ysaías Alvarado |
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| Institution: | aLaboratorio de Protección Vegetal, Centro de Estudios Botánicos y Agroforestales, Instituto Venezolano de Investigaciones Científicas, Calle 74 entre AV 14A y 15, Maracaibo, C.P. 4001, Venezuela;bUnidad de Tecnología Láser y Optoelectrónica, Instituto Zuliano de Investigaciones Tecnológicas, Km11, Carretera Vía La Cañada, Maracaibo, C.P. 4001, Venezuela;cUnidad de Geoquímica, Instituto Zuliano de Investigaciones Tecnológicas, Km11, Carretera Vía La Cañada, Maracaibo, C.P. 4001, Venezuela;dLaboratorio de Electrónica Molecular, Facultad Experimental de Ciencias, Universidad del Zulia, Maracaibo, C.P. 4001, Venezuela;eDepartamento de Investigación y Tecnologías de los Materiales y del Ambiente, Instituto Venezolano de Investigaciones Científicas, Calle 74 entre AV 14A y 15, Maracaibo, C.P. 4001, Venezuela |
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| Abstract: | The use of Fourier transform mid-infrared spectroscopy with attenuated total reflection for characterizing entomopathogenic nematodes is evaluated for the first time. The resulting spectra of Steinernema glaseri and Heterorhabditis indica were compared with the spectrum of Caenorhabditis elegans. In the absorption spectra generated by the nematodes samples, the absorption bands were assigned to the molecular species and some important components were identified including triglycerides, trehalose, glycogen and collagen. Also, the use of star diagrams for the fingerprint section of nematode spectra for separating genera is discussed. |
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| Keywords: | Entomopathogenic nematodes Star diagram Glycogen Trehalose Caenorhabditis elegans Triglycerides |
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