A rapid and simple chemical method for the preparation of Ag colloids for surface-enhanced Raman spectroscopy using the Ag mirror reaction |
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| Institution: | 1. Department of Chemistry, University of Alabama at Birmingham, 901 14th Street South, Birmingham 35294, USA;2. Department of Medicine, Pathology and Pediatrics, University of Alabama at Birmingham, Birmingham 35294, USA;3. Departments of Pathology and Pediatrics, University of Alabama at Birmingham, Birmingham 35294, USA;4. Department of Pediatrics, University of Alabama at Birmingham, Birmingham 35294, USA;1. Center for Computational Natural Sciences and Bioinformatics (CCNSB), International Institute of Information Technology, Hyderabad, Telangana 500 032, India;2. On lien from Department of Physics, Koneru Lakshmaiah Education Foundation, Green Fields, Vaddeswaram, Guntur, Andhra Pradesh 522 502, India;1. Departamento de Física, Universidad del Valle, A.A. 25360, Cali, Colombia;2. Centro de Excelencia en Nuevos Materiales, Universidad del Valle, A.A. 25360, Cali, Colombia;3. Department of Physics, University of Puerto Rico, San Juan, Puerto Rico-00931, USA;4. Institute for Functional Nanomaterials, University of Puerto Rico, San Juan, Puerto Rico-00931, USA;5. Department of Engineering Science and Materials, University of Puerto Rico-Mayaguez Campus, Mayaguez, Puerto Rico-00681, USA;1. Research Center of Ecological Science & Femtosecond Laser Technology, College of Foundation Science, Harbin University of Commerce, Harbin, 150028, China;2. School of Computer and Information Engineering, Harbin University of Commerce, Harbin, 150028, China;3. National Key Laboratory of Science and Technology on Tunable Laser, Harbin Insitute of Technology, Harbin, 150080, China;1. Centre for Heritage Management, Ahmedabad University, Navrangpura, Ahmedabad, 380009, India;2. National Research Laboratory for Conservation of Cultural Property, Aliganj, Lucknow, 226024, India |
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| Abstract: | Colloidal silver (Ag) nanoparticles (AgNP) have been widely used for surface-enhanced Raman spectroscopy (SERS) applications. We report a simple, rapid and effective method to prepare AgNP colloids for SERS using the classic organic chemistry Ag mirror reaction with Tollens’ reagent. The AgNP colloid prepared with this process was characterized using SEM, and the reaction conditions further optimized using SERS measurements. It was found that Ag mirror reaction conditions that included 20 mM AgNO3, 5 min reaction time, and 0.5 M glucose produced AgNP colloids with an average size of 319.1 nm (s.d ± 128.1). These AgNP colloids exhibited a significant SERS response when adenine was used as the reporter molecule. The usefulness of these new AgNP colloids was demonstrated by detecting the nucleotides adenosine 5′-mono-phosphate (AMP), guanosine 5′-monophosphate (GMP), cytidine 5′-monophosphate (CMP), and uridine 5′-monophosphate (UMP). A detection limit of 500 nM for AMP was achieved with the as-prepared AgNP colloid. The bacterium Mycoplasma pneumoniae was also easily detected in laboratory culture with these SERS substrates. These findings attest to the applicability of this AgNP colloid for the sensitive and specific detection of both small biomolecules and microorganisms. |
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| Keywords: | Raman spectroscopy Silver nanoparticles SERS Silver mirror reaction Adenine Mononucleotides Mycoplasma pneumoniae |
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