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阿霉素与牛血清白蛋白结合作用的研究
引用本文:黄波,邹国林,杨天鸣.阿霉素与牛血清白蛋白结合作用的研究[J].化学学报,2002,60(10):1867-1871.
作者姓名:黄波  邹国林  杨天鸣
作者单位:1. 武汉大学生命科学学院,武汉,43002
2. 中南民族大学化学系,武汉,430074
基金项目:国家自然科学基金 (No .3 9770 2 0 0 )资助项目
摘    要:结合荧光光谱和吸收光谱研究了阿霉素与牛血清白蛋白间的结合作用,确定了 阿霉素对牛血清白蛋白的荧光猝灭过程的猝灭机理,测定了不同酸度条件下该结合 反应的结合常数、结合位点数,依据能量转移理认确定了药物和蛋白间的结合距离 。通过比较阿霉素和去糖基阿霉素与牛血清白蛋白的相互作用,结合阿霉素对蛋白 构象的影响,讨论了药物与蛋白的结合模式。

关 键 词:阿霉素  牛血清白蛋白  荧光分光光度法  结合
修稿时间:2002年3月7日

Studies on the Interaction between Adriamycin and Bovine Serum Albumin
HUANG,BoaZOU,Guo-Lin,aYANG,Tian-Mingb.Studies on the Interaction between Adriamycin and Bovine Serum Albumin[J].Acta Chimica Sinica,2002,60(10):1867-1871.
Authors:HUANG  BoaZOU  Guo-Lin  aYANG  Tian-Mingb
Institution:College of Life Sciences, Wuhan University,Wuhan(430072)
Abstract:The binding reaction between adriamycin (ADR) and bovine serum albumin (BSA) was investigated by absorption spectrometry and fluorescence measurement. It is proved that the fluorescence quenching of BSA by ADR is a result of the formation of ADR-BSA complex. The equilibrium constant K and the number of binding sites n were measured at different pH by fluorescence quenching method. The binding distance r and transfer efficiency E between ADR and BSA were also obtained according to Forster theory of non-radiation energy transfer. The effect of ADR on the conformation of BSA was also analyzed using synchronous fluorescence spectroscopy. By studying the binding of 7- deoxyadriamycinone to BSA, the binding mode between ADR and BSA was discussed.
Keywords:adriamycin  7-deoxyadriamycinone  bovine serum albumin  fluo rescence  spectrophotometry
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