| 荧光法研究抗癌药物更生霉素D与小牛胸腺DNA的作用机理 |
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| 引用本文： | 李来生,黄伟东,王瑞琼,鄢远.荧光法研究抗癌药物更生霉素D与小牛胸腺DNA的作用机理[J].化学学报,1999,57(6):572-577. |
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| 作者姓名： | 李来生 黄伟东 王瑞琼 鄢远 |
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| 作者单位： | 南昌大学应用化学研究所,南昌,330047 |
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| 基金项目： | 国家自然科学基金(39660080)，江西省自然科学基金资助项目 |
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| 摘 要： | 以溴化乙锭(ethidiumbromide,EB)为荧光探针,研究了更生霉素D(actinomyinD,ACTD)与小牛胸腺DNA(calfthymusDNA,CTDNA,以下简称DNA)的作用机理。对荧光光谱、荧光偏振、Scatchard图、DNA热变性曲线、DNA盐效应曲线等研究表明,ACTD与DNA之间主要存在嵌入和沟槽两种作用,并证明ACTD与DNA磷酸基之间不存在静电作用。
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| 关 键 词： | 抗癌药 更生霉素D 小牛胸腺 脱氧核糖核酸 溴化乙锭 荧光分光光度法 |
| 修稿时间： | 1998年6月11日 |
Study on the mechanism of the interaction between anticancer drug actinomycin D and calf thymus DNA by fluorescence method |
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| LI Lai-sheng,HUANG Wei-Dong,WANG Rui-Qiong,YAN Yuan.Study on the mechanism of the interaction between anticancer drug actinomycin D and calf thymus DNA by fluorescence method[J].Acta Chimica Sinica,1999,57(6):572-577. |
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| Authors: | LI Lai-sheng HUANG Wei-Dong WANG Rui-Qiong YAN Yuan |
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| Abstract: | The mechanism of the interaction between actionmycin D(ACTD) and calf thymus(CT) DNA has been studied by fluorescence method using ethidium bromide (EB) as a fluorescence probe. The results from varied experiments such as fluorescence spectra,fluorescence polarization, Scatchard curve, DNA heat denaturation, and DNA salt effect etc., support that intercalation and groove binding are the two major modes for the interaction ACTD and CT DNA. The absence of the electrostatic binding between the ACTD and the phosphate backbone of CT DNA has been proved. |
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| Keywords: | actinomycin D calf thymus DNA ethidium bromide fluorescence |
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