血红蛋白的过氧化物酶催化特性研究 |
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引用本文: | 王全林,刘志洪,蔡汝秀,吕功煊.血红蛋白的过氧化物酶催化特性研究[J].化学学报,2003,61(1):34-39. |
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作者姓名: | 王全林 刘志洪 蔡汝秀 吕功煊 |
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作者单位: | 1. 中国科学院兰州化学物理研究所,羰基合成与选择氧化国家重点实验室,兰州,730000 2. 武汉大学化学与环境科学学院,武汉,430072 |
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基金项目: | 中国科学院创新工程、宁夏高等教育科研基金(No.2001-167)资助项目. |
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摘 要: | 探讨了用血红蛋白(Hb)作为辣根过氧化物酶(HRP)的替代物,用于率H2O2氧 化对甲基酚的反应体系,研究了血红蛋白的过氧化物酶特征及其酶催化动力学特性 。稳态速率法测定了米氏常数(Km)、米氏速率(Vm)及反应级数等动力学参数。讨 论了对甲基酚作为血红蛋白的氢供体底物的酶催化反应机理,确立了反应速率方程 。实验比较发现血红蛋白作为过氧化物酶(HRP)的替代物,其催化活性比氯化血 红素(Hemin),β-环糊精-氯化血红素(β-CD-Hemin)等过氧化物模拟酶要高。
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关 键 词: | 过氧化物酶 血红蛋白 酶 特性 荧光 过氧化氢 甲基 酚 P 催化 动力学 |
修稿时间: | 2002年5月9日 |
Study on Peroxidative Characteristics of Hemoglobin |
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Institution: | State Key Laboratory for Oxo Synthesis and Selective Oxidation, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou(730000);School of Chemistry and Environment, Wuhan University, Wuhan(430072);State Key Laboratory for Oxo Synthesis and Selective OxidationLanzhou Institute of Chemical Physics, Chinese Academy of Sciences,Lanzhou(730000) |
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Abstract: | Hemoglobin was used as a substitute of peroxidase in the catalytic oxidation of P-cresol by H_2O_2. The peroxidative characteristics of hemoglobin and enzymatic kinetics were studied. The K_m value (1.85× 10~(-3)mol·L_(-1)) and V_m value (1.6 min_(-1)) were measured by steady-state catalytic velocity at pH=10.3 and temperature 25℃. The reaction mechanism was discussed when p-cresol was used as a hydrogen donor for hemoglobin. The velocity equation has been obtained. The results indicate that the catalytic activity of hemoglobin is higher tan those of other mimic enzymes (e.g. Hemin, β-CD-Hemin). |
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Keywords: | hemoglobin peroxidatic characteristics spectrofluorimetric |
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