丝素蛋白修饰改性的聚羟基脂肪酸酯支架上人体平滑肌细胞的生长

聚三羟基丁酸脂和聚三羟基己酸脂的共聚物(PHBHH_x)是一种具有良好强度和韧性的生物可降解高分子材料, 可作为组织工程心脏瓣膜支架的选择材料之一. 但其生物相容性尚不甚理想. 为此, 本工作利用丝素蛋白修饰改性高分子多孔支架, 以提高支架的生物相容性. 并将人体平滑肌细胞接种在该复合支架上进行体外培养, 以证实改性效果. 其中, 用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐(MTT)方法测试细胞生长, 评估复合支架的细胞相容性. 并用扫描电子显微镜观察细胞在支架上的生长形态. 结果显示, 丝素蛋白修饰改性后的复合支架更有利于细胞的粘附与生长, 平滑肌细胞在支架上表现出良好的生长形态. 这表明, 丝素能够改善多孔支架的生物相容性, 使PHBHH_x/丝素蛋白复合物能更适宜作为组织工程心脏瓣膜的支架材料. 结果对于进一步研究细胞外间质在复合支架上的生长以及体外培养的组织重建有重要的参考意义.

Growth of Human Smooth Muscle Cells on the Silk Fibroin Modified-Polyhydroxyalkanoate Scaffold

In order to find an appropriate scaffold for the tissue engineered heart valve,the poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx),a biodegradable material with a good stress-strain mechanical property,was utilized as a porous scaffold material. But its biocompatibility is not good enough. To improve its biocompatibility,the silk fibroin (SF) was applied to modify the PHBHHx scaffold,and the human smooth muscle cells were seeded on the scaffold in vitro for the demonstration. MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was used to test the cell growths and to evaluate the biocompatibility of the scaffold. SEM was used to observe the cell morphologies. The results reveal that the human smooth muscle cells grow better on the silk fibroin modified hybrid scaffold. It proves that the silk fibroin modification can improve the biocompatibility of the PHBHHx scaffold. The silk fibroin modified hybrid PHBHHx scaffold can be potentially used for the heart valve tissue engineering for further research.