用锇胺分子氢配合物作为探针研究药物与核苷酸的配位 动力学 |
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引用本文: | 杨频,孟祥丽,岳丽君,席小莉.用锇胺分子氢配合物作为探针研究药物与核苷酸的配位 动力学[J].化学学报,2001,59(3):394-404. |
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作者姓名: | 杨频 孟祥丽 岳丽君 席小莉 |
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作者单位: | 山西大学分子科学研究所, |
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摘 要: | 研究了脱氧鸟苷酸(dGMP)及仅有一个配位位点的类似物—咪唑和磷酸氢二钠、磷酸二氢钠与探针的作用,结果表明:dGMP与探针的结合并非咪唑氮和磷酸氧与探针配位结果的简单加和。采用核磁和紫外-停留手段研究了药剂Me~2SnCl~2,Bu~2SnCl~2在不同浓度下与探针竞争dGMP配位位点的溶液化学及其反应动力学,得到了探针和药物与dGMP的结合位点、结合常数以及其动力学结果,对一些反应的机理作出了诠释。
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关 键 词: | 锇胺 脱氧鸟苷酸 咪唑 磷酸氢二钠 核苷酸 探针 |
修稿时间: | 2000年7月19日 |
Kinetic study on the coordination of the agents with nucleotides using Os-H~2 complex as a probe |
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Institution: | Shanxi Univ., Inst of Mol Sci.Taiyuan(030006) |
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Abstract: | Interaction between dGMP or its one-binding-site analogues-imidazole, NaH~2PO~4, Na~2HPO~4 and the probe trans-en~2Os(η^2-H~2)D~2O]^2^+ were studied. The results suggested that the coordination between dGMP and the probe was not the simple sum of the coordination of probe with imidazole and that with NaH~2PO~4 or Na~2HPO~4. The solution chemistry and the dynamics of anticancer agents Me~2SnCl~2, Bu~2SnCl~2 competing for the binding sites of dGMP with the probe at different concentrations were studied using 1^H NMR and UV-stopped flow instruments. Binding sites, equilibrium constants and the dynamic results of the probe and the anticancer agents with dGMP were thus obtained. Some reaction mechanisms is also proposed. |
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Keywords: | dGMP |
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