表面增强拉曼光谱应用于标记免疫多组分检测

作为一种新型的免疫检测方法, 表面增强拉曼光谱(SERS)技术被应用于标记免疫多组分检测. 以多种不同的标记分子(苯硫酚, 联吡啶类分子, 氰基吡啶类分子)分别标记多种不同免疫金溶胶, 通过抗体抗原之间所具有的特异吸附性, 进一步组装“固相抗体-待测抗原-标记免疫金溶胶”多组分三明治复合体系. 利用表面增强拉曼光谱谱峰较窄, 具有较强的分辨率及高灵敏度的特点, 对多种标记分子特征谱峰进行分析判断, 从而识别所加入的多种抗原, 实现SERS标记免疫多组分同时检测的目的, 并对其中氰基吡啶类分子的吸附进行了探讨.

Application of Surface-Enhanced Raman Spectroscopy (SERS) to Multiplex Labelled-immunoassay

As a novel immunoassay measurement, surface-enhanced Raman spectroscopy (SERS) was applied to multiplex labelled-immunoassay. Reporter molecules (thiophenol, bipyridine derivates, cyanopyridine derivates) were attached to different immuno-gold colloids, respectively. By means of the specific adsorption between antigen and antibody, a sandwich structure was constructed, in which labelled immuno-gold colloids and antigen covalently bound to substrate specifically capture different antibodies. SERS has the advantages of narrow band width, high resolution and low detection limit, which can distinguish the characteristic SER spectra of the reporter molecules, then the presence of multifarious antigens was established, and multi-analyte was detected successfully. Also the non-specific adsorption in multiplex labelled-immunoassay and adsorption of cyanopyridine derivates were discussed.