| 巯嘌呤金属配合物与小牛胸腺DNA的作用 |
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| 引用本文： | 卢继新,张贵珠,黄志娜,赵鹏.巯嘌呤金属配合物与小牛胸腺DNA的作用[J].化学学报,2002,60(6):967-972. |
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| 作者姓名： | 卢继新 张贵珠 黄志娜 赵鹏 |
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| 作者单位： | 1. 天津医科大学药学院,天津,300203
2. 南开大学化学系,天津,300071 |
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| 基金项目： | 国家自然科学基金 (N0 .2 95 75 2 0 0 )，天津医科大学自然科学基金资助项目 |
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| 摘 要： | 用溴化乙锭为探针研究了巯嘌呤(mercaptopurine, MP)金属配合物与小牛胸腺 DNA的作用机制，探讨了其作用模式，即巯嘌呤与DNA是非嵌插结合，巯嘌呤金属酴 物与DNA之间的作用为静电方式和一定的嵌入方式。并求得巯嘌呤金属配合物与 DNA的结合常数。
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| 关 键 词： | 巯嘌呤 溴化乙锭 小牛 胸腺 DNA 络合物 |
| 修稿时间： | 2001年10月13 |
Study on the Mechanism of the Interaction between Mercaptopurine Metal Complexes and Calf Thymus DNA |
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| LU,Ji-Xin.Study on the Mechanism of the Interaction between Mercaptopurine Metal Complexes and Calf Thymus DNA[J].Acta Chimica Sinica,2002,60(6):967-972. |
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| Authors: | LU Ji-Xin |
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| Institution: | Department of Pharmacy, Tianjin Medical University.Tianjin(300203);Department of Chemistry, Nankai University.Tianjin(300071) |
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| Abstract: | The mechanism of the interaction between mercaptopurine (MP) metal (Me) complexes and calf thymus (CT) DNA has been studied by using ethidium bromide (EB) as a fluorescence probe. The results from varied experiments such as ultraviolet and fluorescence spectra, fluorescence quenching curve and Scatchard equation and circular dichroism, etc., confirm that intercalation and electrostatic binding are the two major modes for interaction between MP-Me complexs and CT DNA. The absence of the intercalation binding between the MP and CT DNA has been proved. The binding constant of MP-Me complexs with CT DNA has been determined. |
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| Keywords: | metal complex ethidium bromide mercaptopurine DNA |
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