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冷冻过程细胞内水非平衡相变的研究
引用本文:赵刚,刘志峰,杨锐,程曙霞.冷冻过程细胞内水非平衡相变的研究[J].化学学报,2007,65(4):295-299.
作者姓名:赵刚  刘志峰  杨锐  程曙霞
作者单位:1. 中国科学技术大学力学和机械工程系,合肥,230027
2. 中国科学技术大学热科学和能源工程系,合肥,230027
3. 清华大学工程物理系,北京,100084
基金项目:国家自然科学基金(Nos.50506029,50436030)资助项目.
摘    要:引入细胞内冰晶体积项至经典的Mazur方程, 将其适用范围拓宽至可用于描述整个冷冻过程中透过细胞膜的水输运. 通过耦合改进后的Mazur方程、冰晶成核、扩散控制的晶体生长理论, 建立新的胞内冰晶成核与生长的模型. 新模型不受低温保护剂浓度的限制, 可正确预测细胞内冰晶成核、生长、最终体积份额、冰晶大小分布等关键信息. 使用新模型详细研究了甘油初始浓度、降温速率等因素对于冷冻过程中细胞体积变化、胞内溶液冰晶成核和生长过程, 以及最终胞内冰晶体积份额等的影响. 结果表明: 低温保护剂的存在可有效缓解细胞的失水过程, 减少细胞内冰晶的体积份额; 对特定的初始低温保护剂浓度, 存在最佳降温速率对应最小细胞内冰晶体积份额.

关 键 词:胞内冰  成核  冰晶生长  Karlsson模型
收稿时间:2006-6-26
修稿时间:2006-06-262006-11-06

Study on Non-equilibrium Phase Transformation of Intracellular Wa-ter during Freezing Process
ZHAO,Gang,LIU,Zhi-Feng,YANG,Rui,CHENG,Shu-Xia.Study on Non-equilibrium Phase Transformation of Intracellular Wa-ter during Freezing Process[J].Acta Chimica Sinica,2007,65(4):295-299.
Authors:ZHAO  Gang  LIU  Zhi-Feng  YANG  Rui  CHENG  Shu-Xia
Institution:( Department of Modern Mechanics, University of Science & Technology of China, Hefei 230027)( Department of Thermal Science & Energy Engineering, University of Science & Technology of China, Hefei 230027)( Department of Engineering Physics, Tsinghua University, Beijing 100084)
Abstract:The classical water transport model developed by Mazur was extended to be capable of predict- ing cell volumetric change even after intracellular ice formation (IIF).By coupling the modified Mazur model,ice nucleation,and the diffusion-limited ice growth theory,the new model describing IIF and the growth of the intracellular ice (IIG) was developed based on Karlsson's work in 1994.The new model could be used to predict IIF,IIG,the final volumetric fraction of the intracellular ice without the limitation of ini- tial cryoprotective agent (CPA) concentration.The model was then used to study the effect of cooling rate and initial CPA concentration on IIF and IIG.It was found that i) the presence of CPA could markedly slow down IIG and decrease the final volume of intracellular ice,and ii) there exists the optimal cooling rate with minimal final volume of intracellular ice corresponding to certain initial CPA and its concentration.
Keywords:intracellular ice  nucleation  ice crystal growth  Karlsson model
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