国产硅藻土吸附尿激酶机理的研究

在常温下, 尿激酶在浙江土和吉林土表面的吸附等温线分别为V型和II型; 焙烧后两者皆转为III型。吸附等温线类型与硅藻土表面结构、孔结构、表面ζ电位有关。在400℃焙烧的硅藻土等电点值最低, 吸附量最大; 改性后, 吸附量也发生改变。本文还测定了尿激酶在硅藻土表面的吸附形态, 其吸附等温线方程符合0/(1-0)=(Kc)^1/β, 并讨论了平衡常数K和尿激酶吸附功能链段数β随温度的变化。

A study on the mechanism of the adsorption of urokinase on the local diatomite

At normal temperature, the adsorption isotherm of urokinase on Zhejiang and Jiling diatomite surfaces was found to be type V and type II, resp.; both turned into III type after roasting. The adsorption isotherm type is concerned with the surface structure, pore structure, surface z elec. potential of diatomite. When diatomite was roasted at 400? the pI decreased to a min. value and the adsorption amount attained max. value. After the diatomite was modified, the adsorption amount also changed. The adsorption of urokinase on diatomite surface was also determine; its equation was consistent with the q/(1-q) = (Kc)1/b equation and the equilibrium constant K and adsorption function chain segments varying with temperature were discussed.