| 基于核酸脱碱基位点的铽离子荧光增强型单核苷酸多态性识别研究 |
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| 引用本文： | 崔清华,邵勇,马坤,刘桂英,吴飞,许淑娟.基于核酸脱碱基位点的铽离子荧光增强型单核苷酸多态性识别研究[J].化学学报,2011,69(18):2137-2142. |
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| 作者姓名： | 崔清华 邵勇 马坤 刘桂英 吴飞 许淑娟 |
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| 作者单位： | (浙江师范大学物理化学研究所 金华 321004) |
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| 基金项目： | 国家自然科学基金(No.21075112); 浙江省科技厅“钱江人才”计划项目(No.2009R10058); 电分析化学国家重点实验室基金(No.SKLEAC2010001); 教育部留学回国人员科研启动基金资助项目 |
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| 摘 要： | 基于核酸脱碱基(AP)位点构建了无机配体稀土铽离子(Tb3＋)荧光增强型单核苷酸多态性(SNP)识别方法. 在目标链靶标碱基对应的探针链上相应位置引入AP位点, 发现Tb3＋可以选择性地结合在AP位点, 光激发时发生从DNA碱基到结合的Tb3＋的能量转移, 使Tb3＋特征荧光显著增强. 这种荧光增强作用与靶标碱基及AP位点侧翼碱基类型密切相关. 当靶标碱基和侧翼碱基为G时, 荧光最强. 该方法可用于区分肿瘤抑制基因p53密码子177位的C/G碱基变异.
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| 关 键 词： | 脱碱基位点 单核苷酸多态性 铽离子 荧光增强 分子识别 |
Study on Recognition of Single-nucleotide Polymorphism based on Fluorescence Enhancement of Terbium Ion upon Binding at an Abasic Site in DNA |
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| Cui,Qinghua Shao,Yong Ma,Kun Liu,Guiying Wu,Fei Xu,Shujuan.Study on Recognition of Single-nucleotide Polymorphism based on Fluorescence Enhancement of Terbium Ion upon Binding at an Abasic Site in DNA[J].Acta Chimica Sinica,2011,69(18):2137-2142. |
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| Authors: | Cui Qinghua Shao Yong Ma Kun Liu Guiying Wu Fei Xu Shujuan |
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| Institution: | (Institute of Physical Chemistry, Zhejiang Normal University, Jinhua 321004)< |
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| Abstract: | Recognition of single-nucleotide polymorphism(SNP) was proposed in this work on the basis of fluorescence enhancement of an inorganic ligand,terbium ion(Tb3+),upon binding at an abasic(AP) site in DNA.The AP site was deliberately introduced in a probe strand just opposite the base of interest in a target strand.Selective binding of Tb3+ at the AP site was found by observations of its characteristic fluorescence enhancement for AP site-containing DNA by comparison with fully matched DNA without the AP site.T... |
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| Keywords: | abasic stie single-nucleotide polymorphism terbium ion fluorescence enhancement molecu-lar recognition |
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