| 非标记夹心式电化学可卡因适体传感器的研究 |
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| 引用本文： | 上官莉,漆红兰,凌晨.非标记夹心式电化学可卡因适体传感器的研究[J].化学学报,2011,69(18):2196-2200. |
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| 作者姓名： | 上官莉 漆红兰 凌晨 |
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| 作者单位： | 陕西省生命分析化学重点实验室陕西师范大学化学与材料科学学院,西安,710062 |
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| 基金项目： | 国家自然科学基金(No.20805028); 中国博士后科学基金(No.20100471594); 中央高校基本科研业务费专项资金(No.GK201002027)资助项目 |
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| 摘 要： | 设计一种基于双链核酸适体的非标记夹心式电化学适体传感器,建立简单、高灵敏度的可卡因分析方法.首先将末端巯基修饰的捕获适体探针组装在金电极表面,构建可卡因适体传感器.该传感器与目标分子可卡因和部分互补的检测适体探针作用后,在电极表面形成适体/可卡因/适体复合物.以六氨合钌为信号分子,基于单链适体和适体/可卡因/适体复合物...
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| 关 键 词： | 非标记 电化学 传感器 适体 可卡因 |
Label-free and Sandwich Aptamer-based Electrochemical Biosensor for the Determination of Cocaine |
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| Shangguan,Li Qi,Honglan Ling,Chen.Label-free and Sandwich Aptamer-based Electrochemical Biosensor for the Determination of Cocaine[J].Acta Chimica Sinica,2011,69(18):2196-2200. |
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| Authors: | Shangguan Li Qi Honglan Ling Chen |
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| Institution: | Shangguan,Li Qi,Honglan Ling,Chen(Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province,School of Chemistry and Materials Science,Shaanxi Normal University,Xi'an 710062) |
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| Abstract: | A label-free and sandwich aptamer-based electrochemical biosensor for simple and sensitive de-termination of cocaine was developed based on double-strand nucleotide aptamer.Capture ss-DNA was immobilized on gold surfaces by means of self-assembly technique to fabricate cocaine aptamer-based bio-sensor.In the presence of target cocaine and detected aptamer probe,two fragments folded with cocaine and formed a sandwich complex on the electrode surface.Based on the difference binding ability of Ru(NH3)6]3+ to ... |
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| Keywords: | label-free electrochemistry sensor aptamer cocaine |
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