五氯化磷辅助下氨基酸的自组装成肽反应研究

L-α-氨基酸和D-α-氨基酸可与五氯化磷直接发生磷酰化反应，随后自组装成多肽，但β-氨基酸不能成肽，DL-α-氨基酸成肽困难；在SOCl2存在下，α-氨基酸也不能成肽，用电喷雾质谱研究了氨基酸的自组装反应，反应过程中有五元环状的氨基酸五配位磷中间体生成，使用硅烷基保护的氨基酸，在^31PNMR中可观察到五配位磷中间体。

Studies on the self-assembly of amino acids into peptides mediated by phosphorus pentachloride

L-α-Amino acids and D-α-amino acids, mediated by phosphorus pentachloride, could take place the phosphorylation and then assembled itno polypeptides. However, β-amino acids could not assemble into polypeptides, and the self-assembly of DL-α-amino acids into peptides were difficult. α-Amino acids could not assemble into peptides mediated by thionyl chloride. The self-assembly reactions were studied by positive ion electrospray ionization mass spectrometry, and the five-membered cyclic penta-coordinated phosphoryl amino acid intermediates were found in the course of self- assembly into peptides. The intermediates could be trapped by ^31P NMR spectroscopy after amino acids were trimethylsilylized to N, O-bis (trimethylsilyl) amino acids.