| 鲱鱼精DNA修饰纳米金催化Fehling反应——共振散射光谱法检测痕量Hg~(2+) |
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| 引用本文： | 温桂清,李建福,梁爱惠,蒋治良,何星存.鲱鱼精DNA修饰纳米金催化Fehling反应——共振散射光谱法检测痕量Hg~(2+)[J].化学学报,2010,68(1). |
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| 作者姓名： | 温桂清 李建福 梁爱惠 蒋治良 何星存 |
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| 作者单位： | 1. 广西师范大学环境与资源学院,广西环境工程与保护评价重点实验室,桂林,541004
2. 桂林工学院材料与化学工程系,桂林,541001 |
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| 基金项目： | 广西自然科学基金(Nos.0728213,0832260,0991021Z);;广西环境工程与保护评价重点实验室基金资助项目 |
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| 摘 要： | 用鲱鱼精DNA(hsDNA)修饰10nm的纳米金制备了Hg2+的hsDNA修饰纳米金共振散射光谱探针(AuhsDNA).在pH7.0Tris-HCl缓冲溶液中及0.017mol/LNaCl存在下,Hg2+与AuhsDNA形成稳定的Hg2+-DNA结合物,引起AuhsDNA中的纳米金析出并聚集形成纳米金簇.该溶液用150nm滤膜过滤后,滤液中过量的AuhsDNA可催化Fehling试剂-葡萄糖反应生成氧化亚铜微粒,该微粒在580nm处有一个较强的共振散射峰.随着汞离子浓度增大,形成的纳米金簇越多,滤液中AuhsDNA越少,生成的氧化亚铜微粒减少,580nm处氧化亚铜微粒的共振散射光强度线性降低,其共振散射光强度降低值ΔI580nm与汞离子浓度在1～833nmol/L范围内成线性,回归方程、相关系数、检出限分别为ΔI580nm2+Hg=0.37C+0.9,0.9990,0.3nmol/LHg2+.该法用于废水中Hg2+的检测.
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| 关 键 词： | 汞离子 hsDNA修饰纳米金探针 纳米催化 共振散射光谱法 |
Resonance Scattering Spectral Detection of Trace Hg~(2+) Using Herring Sperm DNA Modified Nanogold as Catalyst of the Fehling Reaction |
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| Wen,Guiqing,Li,Jianfu,Liang,Aihui,Jiang,Zhiliang,He,Xingcun.Resonance Scattering Spectral Detection of Trace Hg~(2+) Using Herring Sperm DNA Modified Nanogold as Catalyst of the Fehling Reaction[J].Acta Chimica Sinica,2010,68(1). |
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| Authors: | Wen Guiqing Li Jianfu Liang Aihui Jiang Zhiliang He Xingcun |
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| Institution: | a Guangxi Key Laboratory of Environmental Engineering;Protection and Assessment;Guangxi Normal University;Guilin 541004;b Guilin University of Technology;Guilin 541004 |
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| Abstract: | Herring sperm DNA (hsDNA) was used to modify 10 nm nanogold to obtain a resonance scattering (RS) spectral probe (AuhsDNA) for Hg2+.In the medium of pH 7.0 Tris-HCl-0.017 mol/L NaCl,Hg2+ interacted with AuhsDNA to form stable Hg2+-hsDNA complex and larger nanogold clusters,which could be removed by membrane filtration.The excess AuhsDNA in the filtration solution exhibited catalytic effect on the Fehling reagent-glucose reaction to form Cu 2 O particles that exhibit the strongest RS peak at 580 nm.With addi... |
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| Keywords: | Hg2+ herring sperm DNA modified nanogold probe nanocatalysis resonance scattering |
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