丁二酸二异辛酯磺酸钠(AOT)/TritonX-100混合反胶束体系中纤维素酶降解纤维素的研究

对于底物不溶于水的纤维素降解反应而言,为了增强纤维素酶的活性,在丁二酸二异辛酯磺酸钠(AOT)/异辛烷反胶束体系中加入非离子表面活性剂TritonX-100进行纤维素降解实验.结果表明,在AOT中加入非离子表面活性剂TritonX-100可以使纤维素酶的活性提高,非离子表面活性剂TritonX-100与AOT的最佳物质的量之比是0.20.考察了水与表面活性剂的物质的量之比(Wo)、不同酸度(pH)和不同温度(T)等其他反应条件对纤维素降解反应的影响.研究结果表明,反应的最佳条件是:Wo为3.3,T为315.11K,pH为5.10.

Study on Enzymatic Degradation of Cellulose in Diisooctyl Succinate Sodium Sulfonate (AOT)/Triton X-100 Mixed Reverse Micellar System

To enhance the cellulase activity for the reaction of water-insoluble substrates, the diisooctyl succinate sodium sulfonate (AOT)/isooctane reverse micellar was modified by co-adsorption of Triton X-100. An appreciable increase of cellulase activity was observed and the results show that the optimal molar fraction of AOT/Triton X-100 is 0.20. The influences of other reaction conditions on the molar ratio of water to total surfactants (Wo), different pH and temperature have been investigated, and the optimal conditions for the reaction were Wo=3.3, T=315.11 K, pH=5.10.