| 两种荧光探针法研究三种非甾体抗炎药与DNA的相互作用 |
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| 引用本文： | 杨昌英,刘义,曾芳,李静,李强国,李林尉.两种荧光探针法研究三种非甾体抗炎药与DNA的相互作用[J].化学学报,2007,65(18):2076-2080. |
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| 作者姓名： | 杨昌英 刘义 曾芳 李静 李强国 李林尉 |
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| 作者单位： | (1武汉大学化学与分子科学学院 武汉 430072)(2三峡大学化学与生命科学学院 宜昌 443002)(3病毒学国家重点实验室 武汉 430072) |
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| 基金项目： | 国家自然科学基金
,
湖北省创新研究群体科学基金
,
教育部创新团队发展基金
,
国家自然科学基金 |
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| 摘 要： | 采用溴化乙锭(EB)和中性红(NR)两种荧光探针分别考察了三种非甾体抗炎药(吲哚美辛、舒林酸和托美丁)与DNA的相互作用, 采用荧光发射光谱和共振散射光谱技术得出了基本一致的结论: 吲哚美辛和舒林酸可以在一定程度上与DNA结合(舒林酸更强), 结合方式可能有嵌插作用, 也可能有药物分子在DNA双链表面的组装, 作用力来源于静电作用. 而托美丁几乎不与DNA作用, 因为其疏水性弱, 与DNA分子结合几率小.
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| 关 键 词： | 非甾体抗炎药 DNA 荧光发射光谱 共振散射光谱 探针 |
| 收稿时间： | 2006-8-28 |
| 修稿时间： | 2006-08-28 |
Interaction of Three Kinds of Non-steroidal Anti-inflammatory Drugs with DNA Investigated by Two Fluorescence Probes |
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| YANG,Chang-Ying,LIU,Yi,ZENG,Fang,LI,Jing,LI,Qiang-Guo,LI,Lin-Wei.Interaction of Three Kinds of Non-steroidal Anti-inflammatory Drugs with DNA Investigated by Two Fluorescence Probes[J].Acta Chimica Sinica,2007,65(18):2076-2080. |
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| Authors: | YANG Chang-Ying LIU Yi ZENG Fang LI Jing LI Qiang-Guo LI Lin-Wei |
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| Institution: | (1 College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072)(2 College of Chemistry and Life Science, Three Gorges University, Yichang 443002)(3 State Key Laboratory of Virology, Wuhan 430072) |
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| Abstract: | The interaction of three chosen non-steroidal anti-inflammatory drugs (NSAID), indomethacin, sulindac and tolmetin with calf thymus DNA has been investigated by fluorescence with the use of fluores- cence probes, ethidium bromide (EB) and neutral red (NR). When bound to DNA, sulindac and indometha- cin could quench the fluorescence of EB at low concentration by exchanging with the EB probe, and attained saturation quickly. But sulindac and indomethacin continuously quenched the fluorescence emission and resonance light scattering spectra of NR in the presence of DNA until rather high concentration. These spec- tral characteristics supported the intercalation of NSAID into DNA as well as electrostatic assembly on the surface of DNA double strands. |
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| Keywords: | non-steroidal anti-inflammatory drug calf thymus DNA fluorescence resonance light scattering spectra probe |
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