单糖立体异构体的测定: 正丁基硼酸衍生物的快原子轰击质谱

研究了把衍生化反应引用到正离子质谱中, 利用正丁基硼酸n-BuB(OH)2]对自然界常见的11种单糖: D-核糖、D-来苏糖、D-木糖、L-阿拉伯糖、L-鼠李糖、L-岩藻糖、D-葡萄糖、D-甘露糖、D-半乳糖、D-果糖、L-山梨糖进行衍生化反应, 通过快原子轰击(FAB)谱图区分单糖的立体异构体, 以进一步探讨区分寡糖、多糖及糖甙化合物中糖的立体异构体的方法.

Differentiation of stereoisomeric monosaccharides as n-butaneboronate derivatives by fast atom bombardment mass spectrometry

The stereoselective reaction of 11 monosaccharides D-ribose, D-lyxose, D-xylose, L-arabinose, L-rhamnnose, L-fucose, D-glucose, D-mannose, D-galactose, D-fructose, L-sorbose with butaneboronic acid was studied by fast atom bombardment mass spectrometry (FAB MS). Characteristic mass spectra of the derivatized monosaccharides showed some differences which could be used in the differentiation of the stereoisomers in FAB MS. This reaction was effected by the orientation of OH groups and the favored configuration (furanose or pyranose) of monosaccharides, 1-hydroxyl groups also participated in the reaction if 2 and 3-OH groups are in the trans-positions.