首页 | 本学科首页   官方微博 | 高级检索  
     检索      

巨大芽孢杆菌D01吸附金(AU3+)的谱学表征
引用本文:林种玉,吴剑鸣,傅博强,薛茹,周剑章,郑泉兴,刘月英,傅锦坤.巨大芽孢杆菌D01吸附金(AU3+)的谱学表征[J].化学学报,2004,62(18):1829-1834.
作者姓名:林种玉  吴剑鸣  傅博强  薛茹  周剑章  郑泉兴  刘月英  傅锦坤
作者单位:1. 厦门大学化学系,固体表面物理化学国家重点实验室,厦门,361005
2. 厦门大学化学系,生物系,厦门,361005
摘    要:对休眠的巨大芽孢杆菌(Bacillus megatherium)D01菌体吸附Au^3 的作用过程进行了谱学表征.运用AAS考察了pH、时间和温度对D01菌体吸附Au^3 过程的化学动力学和热力学相关参数的影响.D01菌粉中硫元素含量的EDX分析说明该菌体中对Au^3 具有还原作用的L-半胱氨酸和蛋氨酸的含量极少;D01菌体水解后葡萄糖含量的UV-vis测定说明该菌体水解产物中含有一定量的还原糖,空白的和吸附Au^3 的D01菌体的FFIR检测表明该菌体细胞壁肽聚糖层糖类化合物的羟基和肽链侧链氨基酸残基离子化羧基为吸附Au^3 的活性基团;肽聚糖层部分多糖的水解产物低聚糖、二糖及单糖等还原糖的半缩醛羟基游离态醛基为电子供体,将Au^3 原位还原成Au^0.葡萄糖和Au^3 相互作用的XRD和FFIR表征证明Au^3 是在还原糖的醛基上直接被还原成Au^0.

关 键 词:巨大芽孢杆菌  生物吸附  金离子  谱学  化学动力学  热力学  生物还原

Spectroscopic Characterization on Interaction of Gold (Au3+) Biosorption by Bacillus megaterium DO1
LIN,Zhong-Yu,a WU,Jian-Minga FU,Bo-Qianga XUE,Rua ZHOU,Jian-Zhanga ZHENG,Quan-Xinga LIU,Yue-Yingb FU,Jin-Kuna.Spectroscopic Characterization on Interaction of Gold (Au3+) Biosorption by Bacillus megaterium DO1[J].Acta Chimica Sinica,2004,62(18):1829-1834.
Authors:LIN  Zhong-Yu  a WU  Jian-Minga FU  Bo-Qianga XUE  Rua ZHOU  Jian-Zhanga ZHENG  Quan-Xinga LIU  Yue-Yingb FU  Jin-Kuna
Abstract:Biosorptive interaction of gold with resting cell of Bacillus megatherium D01 biomass has been characterized by spectroscopic techniques. The effects of pH, time and temperature on the correlation parameters of chemical kinetics and thermodynamics of the binding reaction has been investigated through the determination of the gold ion binding to the biomass using atomic absorption spectrophotometry (AAS). The analysis for sulfur content in dry powder of the D01 biomass by energy dispersive X-ray (EDX) shows that cysteine and methionine being capable of reducing Au 3+ to Au0 is very small, whereas the glucose content in hydrolysates of the biomass analyzed by ultraviolet-visible spectrophotometry (UV-vis) indicates that the amount of the reducing sugars in the biomass is much larger than 3.33%. The Fourier transform infrared (FTIR) spectroscopy on blank and gold-loaded biomass demonstrates that the active groups such as the hydroxyl group of saccharides and the ionized carboxyl group of amino acid residues on the cell wall seem to be the sites for Au 3+ binding, and the free aldehyde group of the hemiacetalic hydroxyl group from reducing sugars, i.e. the hydrolysates of the polysaccharides, serving as the electron donor, in situ reduces the Au 3+ to Au0. X-ray powder diffractometry (XRD) and FTIR spectroscopic characterizations of the interaction of Au 3+ with glucose confirm that the reduction of Au 3+ to Au0 can directly occur in the aldehyde group of the reducing sugars.
Keywords:biosorption  Bacillus megatherium  gold  bioreduction  FTIR
本文献已被 CNKI 维普 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号