有序中孔炭/四氧化三铁复合材料的制备及其用于血红蛋白的电化学研究

采用微湿含浸法制备了有序中孔炭/四氧化三铁磁性材料.采用透射电镜和X射线衍射对复合材料进行了表征.将血红蛋白(Hb)固定于材料表面,对其直接电化学行为进行了研究,结果表明Hb在该材料内仍保持了其生物活性,在pH=7.0的PBS缓冲液中,血红蛋白表现出一对峰形良好的准可逆氧化还原峰,为Hb的Fe(Ⅲ)/Fe(Ⅱ)电对的特征峰,求出式电位E0’为-0.306 V,电子转移数为n=1.226,电荷传递系数为α=0.51,表观异相电子转移速率常数为KS=0.0144s-1.在3.00×10-6到1.50×10-4mol/L浓度范围内,血红蛋白的浓度与其响应电流呈良好的线性关系,线性相关系数为R=0.9924,最低检测限为0.270×10-6mol/L.

Preparation of the Ordered Mesoporous Carbon/Fe3O4 and Its Application for the Direct Electrochemistry of Hemoglobin

The novel magnetic composites of ordered mesoporous carbon/Fe3O4 were prepared with a micro-wetness impregnation method.The composites were characterized by transmission electron microscope and X-ray diffraction.Hemoglobin was immobilized onto the surface of the composites,and was shown to keep its biological activity well.The electrochemical behavior of Hb was studied,and a pair of well-defined,quasi-reversible cyclic voltammetric redox peaks was obtained,which was characteristics of Hb Fe(Ⅲ)/Fe(Ⅱ) redox couples.The formal potential(E0’),the electron transfer number(n),and transfer coefficient(α) were calculated to be-0.306 V,1.226 and 0.51,respectively.The average apparent heterogeneous electron transfer rate constant(KS) obtained for Hb was 0.0144 s-1.Linear sweep voltammetric measurement of Hb indicated that the peak currents of Hb were linear to its concentration ranging from 3.00×10-6to 1.50×10-4mol/L with the detection limits of 0.270×10-6mol/L.