秋水仙碱水解产物与某些酸性磺酞类染料相互作用的 共振瑞利散射光谱及其分析应用

在pH 2.9～4.6 Britton-Robinson (BR)缓冲溶液中, 秋水仙碱的水解产物(H-COL)能与溴酚蓝(BPB)、溴甲酚绿(BCG)、溴百里酚蓝(BTB)和百里酚蓝(TB)等酸性磺酞类染料(ASPD)反应形成1∶1的离子缔合物, 此时将引起共振瑞利散射(RRS)的急剧增强, 并产生新的RRS光谱. 秋水仙碱水解产物与溴酚蓝、溴甲酚绿、溴百里酚蓝和百里酚蓝形成离子缔合物的最大散射波长分别位于327, 311, 305和306 nm处. 散射增强(ΔI)与秋水仙碱浓度在一定范围内成正比, 不同体系对于秋水仙碱的检出限(3σ)分别为12.3, 15.1, 16.4和20.0 ng•mL－1 (TB). 研究了适宜的反应条件, 考察了共存物质的影响, 表明方法有较好的选择性. 基于秋水仙碱水解产物与酸性磺酞类染料离子缔合物的反应, 发展了一种较灵敏, 且简便、快捷测定秋水仙碱的新方法. 方法用于片剂、黄花、血清和尿样中秋水仙碱的测定, 获得了满意的结果.

Resonance Rayleigh Scattering Spectra of Interaction of the Hydrolyzed Products from Colchicine with Some Acid Sulfonephathalein Dyes and Their Analytical Application

In pH 2.9～4.6 Britton-Robinson (BR) buffer solution, the hydrolyzed product of colchicine was reacted with some acid sulfonephthalein dyes such as bromophenol blue (BPB), bromocresol green (BCG), bromothymol blue (BTB) and thymol blue (TB) etc. to form ion-association complexes. As a result, the resonance Rayleigh scattering (RRS) was enhanced greatly, and a new RRS spectrum appeared. The maximum RRS signals of four ion-association complexes are located at 327, 311, 305 and 306 nm, separately. The intensities of RRS are proportional to the concentration of colchicine. And the detection limits for colchicine are 12.3, 15.1, 16.4 and 20.0 ng•mL－1, respectively. The optimal conditions and the effect factors of the reaction were investigated. A more sensitive, simple and fast method for the determination of colchicine based on the RRS technique has been developed, which was applied to determination of colchicine in tablet, hemerocallis, serum and urine samples with satisfactory results.