氯化铕对大黄愈伤组织生长及超微结构的影响

以掌叶大黄的愈伤组织为材料，研究了不同浓度氯化铕对其生长的影响，并利用透射电镜技术研究了氯化铕对大黄细胞超微结构的影响。实验结果表明，低浓度氯化铕促进愈伤组织生长，高浓度则抑制生长，浓度达到１００ｍｇ／Ｌ则可导致大黄外植体全部死亡。氯化铕对大黄的促进作用可被钙调蛋白的拮抗剂氯丙嗪抑制。１０ｍｇ／Ｌ氯化铕可使大黄愈伤组织的细胞形态发生明显变异。

Effect of Europium Chloride on Callus Growth and Cell Ultrastructure of Rheum

Effect of europium chloride on the callus growth and cell ultrastructure of Rheum was studied. The effect of europium chloride on the growth of the callus was a biphasic one, with stimulation at low concentrations, and inhibition at higher concentrations. The result shows that explants of Rheum will die when the concentration of europium chloride is 100 mg/L. The stimulate effect of europium chloride can be inhibited by anti calmodulin drug chlorpromazine. 10 mg/L of europium chloride causes variation of the cell ultrastructure.