| 尖吻蝮蛇蛇毒中纤溶组分与铽的相互作用 |
| |
| 引用本文： | 王守业,丁兰.尖吻蝮蛇蛇毒中纤溶组分与铽的相互作用[J].中国稀土学报,1999,17(1):1-6. |
| |
| 作者姓名： | 王守业 丁兰 |
| |
| 作者单位： | 中国科学技术大学化学系!合肥，230026，中国科学院长春应用化学研究所稀土化学与物理开放实验室!长春，130022，中国科学院长春应用化学研究所稀土化学与物理开放实验室!长春，130022，中国科学技术大学化学系!合肥，230026，中国科学院长春应用化学研究所稀土化学与物理开放实验室! |
| |
| 基金项目： | 国家自然科学基金!29471027，中国科学院长春应用化学所稀土化学与物理开放实验室基金 |
| |
| 摘 要： | 利用平衡析，原子吸收，荧光滴定和荧光寿命测定等方法确定了皖南尖吻蝮蛇蛇毒纤溶组分中Ｃａ＾２＋含量，并利用荧光光谱研究了Ｔｂ＾３＋与ＦＰ中色氨酸残基之间能量转移和ＦＰ中的微区结构。
|
| 关 键 词： | 铽 纤溶组分 能量转移 尖吻蝮蛇蛇毒 蛇毒 |
| 修稿时间： | 19971224 |
Intraction between Tb~(3 ) and Fibrinolytic Principle from Agkistrodon Acutus Venom |
| |
| Wang Shouye, Ding Lan, Liu Qingliang, Zhao Daqing, Chen Zhenlong,Zhang Zude.Intraction between Tb~(3 ) and Fibrinolytic Principle from Agkistrodon Acutus Venom[J].Journal of the Chinese Rare Earth Society,1999,17(1):1-6. |
| |
| Authors: | Wang Shouye Ding Lan Liu Qingliang Zhao Daqing Chen Zhenlong Zhang Zude |
| |
| Abstract: | By using equilibrium dialysis, atomic absorption spectrometry, fluorescence titration anddetermination of fluorescence lifetime, it can be followed that each fibrinolytic principle(FP)molecule contains one Ca2 -binding site and one Ca2 . Energy transfer between Tb3 and theTrp residue in FP was studied through fluorescence spectroscopy. The results show that theCa2 in a FP molecule can be substitute by a Tb3 . In FP molecule, the excition energy can betransferred from the Trp residue as an energy donor to the Tb3 as an acceptor, and substitutedinto FP and located near the Trp residue. The distance between Tb3 and the Trp residue, a0.375 urn, is worked out with the experimental data and Forster theory. |
| |
| Keywords: | Rare earths Terbium Fibrinolytic principle Energy transfer |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
