铈-明胶多孔支架的制备研究

采用一种新型搅拌发泡冷冻干燥法制备铈-明胶组织工程多孔支架,并对其吸水性能及抑菌活性进行了研究。结果表明,与目前通用的冷冻干燥方法相比,搅拌发泡冷冻干燥法制备的明胶多孔支架,孔连通性好,吸水率较高,并且避免了表面皮层的形成;支架中相互连通的大孔有利于细胞透过、生长与繁殖,且支架壁上的小孔有利于营养物质的输送。铈-明胶多孔支架对金黄色葡萄球菌的抑菌活性显著,且随着铈浓度的增加,其抑菌活性逐渐增大。

Preparation of Porous Cerium Nitrate-Gelatin Scaffolds

Stirring-foaming freeze-drying technic,as a novel preparation method of cerium nitrate-gelatin tissue engineering scaffolds were proposed,and the biophysical properties of these cerium nitrate-gelatin scaffolds were studied in details.Compared with the traditional technic of freeze-drying,the presented technic can greatly enlarge the average pore size and water-absorbability of the scaffolds.Besides,the interconnected large pores were beneficial to cells penetration,propagation and adhesion and the minor pores were beneficial to nutrients delivery.In addition,the connectivity of the pores was improved,and the surface skin,which is a problem in traditional method,can be avoided in this study.It was found that the bacteriostatic activity of cerium nitrate-gelatin scaffolds against Staphylococcus aureus was outstanding,and increased with the increase in the cerium nitrate concentration.