硝酸镧对大鼠肝脏的亚慢性毒性实验研究

以大鼠为对象，连续用生理盐水和20，10，2，0．2，0．1mg.kg^-1La(NO3)3灌胃6个月后，观察了体重，肝体比变化；检测了血清中谷草转氨酶，谷丙转氨酶，碱性磷酸酶和δ－谷氨酰转移酶含量的变化，应用组织化学及透射电镜技术观察了不同剂量La(NO3)3对大鼠肝脏形态结构的影响。20mg/kg^-1La(NO3)3组动物体重增长缓慢，0．1mg.kg^-1La(NO3)3组动物体重增加较快；20mg/kg^-1La(NO3)3组肝脏汇管区有炎细胞浸润，有些肝细胞内有少量的脂滴，糖原减少，线粒体基质密度增高，有电子密度高的致密体，肝细胞核形态正常或轻度变形。

Subchronic Toxicity of Lanthanum Nitrate on Liver of Rats

Young wistar rats were divided into six groups， to which they were given La(NO3)3 at 20， 10， 2， 0.2， 0.1 mg*kg－1 and the control group with physiological saline respectively for six months. The weight and the ratio of the liver to body weight were accounted. Pathological changes of liver were observed by optical microscope and transmission electron microscope. The white blood cells(WBC) were classified and counted. Glutamic-oxalacetic transaminase (GOT), glutamic-pyruvic transitanase (GPT)， gamma-glutamyl transferase (γ-GT) and alkline phosphatase (ALP) in the serum were measured. The results indicate that the body weight of animals increases slowly in the group of 20 mg.kg－1 La(NO3)3, but it grows quickly in the rats fed with 0.1 mg.kg－1 La(NO3)3. The classification and count of WBC compared with control group are not varied between female and male rats. Biochemical indexes are no abnormal changes. In the group of 20 mg.kg－1 La(NO3)3, there are the lipid droplets and decrease of glycogen in the hepatocytes, denser matrix of the mitochondria, deformation of the nucli of some hepatocytes with different degrees and infiltration of inflammatory cells at portal area. The more the rats are given doses, the more the number of bodies contain high electronic dense gravel-like granules and the secondary lysosomes with dense bodies. The rats fed with 20 mg.kg－1 La(NO3)3 for six months shows injurious effects on the hepatocytes.