| 过硫酸钾氧化吸光光度法测定植物总氮 |
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| 引用本文： | 吴建之,葛滢,王晓月.过硫酸钾氧化吸光光度法测定植物总氮[J].理化检验(化学分册),2000,36(4):166-167. |
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| 作者姓名： | 吴建之 葛滢 王晓月 |
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| 作者单位： | 1. 浙江大学分析测试中心,杭州,3l0028
2. 浙江大学生物系 |
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| 摘 要： | 提出植物样经Ｈ２ＳＯ４＋Ｈ２Ｏ２凯氏消煮，试液中和后用过硫酸钾碱性溶液氧化，在酸性条件下用紫外吸光光度法测定包括铵态氮、硝态氮及亚硝态氮的总氮含量。对植物标准物质ＧＢＷ０７６０３作平行试验，分析结果与标准值的相对误差为４．７％；方法的相对标准偏差为３．３％。用该法作了批量分析，操作简便，数据可靠。
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| 关 键 词： | 植物 总氮 紫外吸光光度法 过硫酸钾 |
| 修稿时间： | 1998-12-29 |
UV ABSORPTIOPHOTOMETRIC DETERMINATION OF TOTAL NITROGEN IN PLANT AFTER K2S2O8 OXIDATION |
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| Wu Jianzhi,Ge Ying,Wang Xiaoyue.UV ABSORPTIOPHOTOMETRIC DETERMINATION OF TOTAL NITROGEN IN PLANT AFTER K2S2O8 OXIDATION[J].Physical Testing and Chemical Analysis Part B:Chemical Analgsis,2000,36(4):166-167. |
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| Authors: | Wu Jianzhi Ge Ying Wang Xiaoyue |
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| Institution: | Wu Jianzhi
;(Centre of Analysis and Detection, Zhejiang University Hangzhou 310028);Ge Ying
,Wang Xiaoyue
;(Dept. Of Biology, Zhejiang University) |
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| Abstract: | The determination of total nitrogen in plant by ultra violet absorptiophotometric method was proposed in this paper. The plant sample was digested by the Kjeldahl method with H 2SO 4 H 2O 2. The solution of sample after neutralization was oxidized by alkaline K 2S 2O 8 solution, and it was used to determine the total nitrogen content including amino, nitric and nitrous nitrogen. The proposed method was checked with the plant standard reference material (GBW07603), and a relative error of 4.7% was found between the results obtained and the standard value given. The RSD of the present method is 3.3%. The batch analysis of plant samples by this method was proved to be easy to operate, and to give accurate results. |
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| Keywords: | Plant Total nitrogen UV absorptiophotometric method |
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