| 高效液相色谱法测定发酵液中杆菌肽A |
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| 引用本文： | 张玉明,倪志华,李琳,李宝库.高效液相色谱法测定发酵液中杆菌肽A[J].理化检验(化学分册),2011(5). |
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| 作者姓名： | 张玉明 倪志华 李琳 李宝库 |
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| 作者单位： | 河北大学生命科学学院;河北大学药学院; |
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| 基金项目： | 河北大学自然科学基金青年项目(2008Q49) |
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| 摘 要： | 提出了高效液相色谱法测定发酵液中杆菌肽A含量的方法。地衣芽孢杆菌发酵液经离心得到杆菌肽A的粗提液。所得粗提液再经三氯乙酸提取,离心分离。取上清液用Beckman C18色谱柱分离,用乙腈和磷酸二氢钾缓冲溶液以不同体积比混合为流动相梯度洗脱,在220 nm波长处进行测定。杆菌肽A的质量浓度在1.0~15.0 g·L-1范围内与其峰面积呈线性关系,检出限(3S/N)为0.08 g·L-1。在7.37 g·L-1和11.01 g·L-1两个添加水平做回收试验,平均回收率分别为98.1%和97.4%。
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| 关 键 词： | 高效液相色谱法 杆菌肽A 地衣芽孢杆菌 发酵液 |
HPLC Determination of Bacitracin A in Fermentation Liquor |
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| ZHANG Yu-ming,NI Zhi-Hua,LI Lin,LI Bao-ku.HPLC Determination of Bacitracin A in Fermentation Liquor[J].Physical Testing and Chemical Analysis Part B:Chemical Analgsis,2011(5). |
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| Authors: | ZHANG Yu-ming NI Zhi-Hua LI Lin LI Bao-ku |
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| Institution: | ZHANG Yu-ming1,NI Zhi-Hua1,LI Lin1,LI Bao-ku2(1.College of Life Science,Hebei University,Baoding 071002,China,(2.College of Pharmacy,China) |
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| Abstract: | HPLC was applied to the determination of bacitracin A in fermentation liquor.The crude extract obtained by centrifuging fermentation liquor of bacillus licheniformis,was extracted with trichloroacetic acid.After centrifugation,the supernatant was separated by gradient elution on Bechman C18 column with mixtures of acetonitrile and potassium dihydrogen phosphate buffer solution in various ratios as mobile phase.Wavelength of 220 nm was chosen for UV-detection for bacitracin A.Linear relationship between valu... |
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| Keywords: | HPLC Bacitracin A Bacillus licheniformis Fermentation liquor |
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