| 高效液相色谱-质谱法及模式识别法用于鉴别普洱生茶与熟茶 |
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| 引用本文： | 倪倩,佟玲,高钧,李文博,王强.高效液相色谱-质谱法及模式识别法用于鉴别普洱生茶与熟茶[J].理化检验(化学分册),2012(2):171-174,181. |
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| 作者姓名： | 倪倩 佟玲 高钧 李文博 王强 |
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| 作者单位： | 中国药科大学;天士力制药股份有限公司研究院 |
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| 摘 要： | 采用高效液相色谱-质谱联用技术及高效液相色谱法对生熟普洱茶中的主要成分进行定性和定量分析。鉴定出普洱茶水溶液中8种主要成分,分别为没食子酸(GA)、没食子酸儿茶素(GC)、表没食子酸儿茶素(EGC)、儿茶素(C)、咖啡因(CAF)、表儿茶素(EC)、表没食子酸儿茶素没食子酸酯(EGCG)和表儿茶素没食子酸酯(ECG)。以这8种成分的含量为指标,对普洱生茶和熟茶各20批进行主成分分析、聚类分析和判别分析,能准确地区分普洱生茶与熟茶。
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| 关 键 词： | 普洱生茶 普洱熟茶 高效液相色谱法 高效液相色谱-质谱联用 聚类分析 主成分分析 判别分析 |
Identification of Raw and Ripe Pu-Er Tea by HPLC-MS/MS with Pattern Recognition |
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| NI Qian,TONG Ling,GAO Jun,LI Wen-bo,WANG Qiang.Identification of Raw and Ripe Pu-Er Tea by HPLC-MS/MS with Pattern Recognition[J].Physical Testing and Chemical Analysis Part B:Chemical Analgsis,2012(2):171-174,181. |
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| Authors: | NI Qian TONG Ling GAO Jun LI Wen-bo WANG Qiang |
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| Institution: | 1*(1.China Pharmaceutical University,Nanjing 210009,China; 2.Institute of Pharmaceutical Analysis,Tianjin Tasly Pharmacy Group Co.,Ltd.,Tianjin 300402,China) |
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| Abstract: | HPLC-MS/MS and HPLC were applied to the qualitative and quantitative analysis of raw and ripe Pu-Er tea.Eight main components were identified;they were gallic acid(GA),gallocatechin(GC),epigallocatechin(EGC),catechin(C),caffeine(CAF),epicatechin(EC),epigallocatechin gallate(EGCG) and epicatechin gallate(ECG).The contents were used as characteristic indexes for the identification of 20 batches each of the raw and ripe tea by pattern recognition techniques,including principal component analysis(PCA),clustering analysis(CA) and linear discriminant analysis(LDA).The proposed method was useful for distinguishing between raw and ripe Pu-Er tea. |
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| Keywords: | Raw Pu-Er tea Ripe Pu-Er tea HPLC HPLC-MS/MS Cluster analysis Principal component analysis Linear discriminant analysis |
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