高效液相色谱法测定染发剂中2-硝基-p-苯二胺

应用高效液相色谱法测定了染发剂中2-硝基-p-苯二胺的含量。样品用乙醇-水(1+1)混合溶液提取后,经水系C18色谱柱分离,以(A)10mol·L-1柠檬酸缓冲溶液-10mol·L-1辛烷磺酸钠溶液(pH 2.6)与(B)乙腈以体积比75比25组成的混合溶液为流动相进行等度洗脱,用二极管阵列检测器于410nm检测。2-硝基-p-苯二胺的线性范围为2.4～240mg·L-1,检出限(3S/N)为0.7mg·L-1。以染发剂样品为基体,进行加标回收试验,回收率在90.4%～99.4%之间,相对标准偏差(n=6)在0.35%～3.0%之间。

HPLC Determination of 2-Nitro-p-benzenediamine in Hair Dyes

HPLC was applied to the determination of 2-nitro-p-benzenediamine in hair dyes.The sample was extracted with mixture of CH3CH2OH-H2O(1+1).The extract was separated on auqo-C18 column by gradient elution with eluants containing A(10 mol·L-1 citric acid +10 mol·L-1 sodium octanesulfonate of pH 2.6) and B(acetonitrile) mixed in the ratio of 75 to 25,and determined with diode array detector at the wavelengths of 410 nm.Linearity range for 2-nitro-p-benzenediamine was found between 2.4-240 mg·L-1,with detection limit(3S/N) of 0.7 mg·L-1.Using sample of hair dyes as matrix,recovery test was made by standard addition method,values of recovery found were in the range of 90.4%-99.4%,with RSD′s(n=6) in the range of 0.35%-3.0%.