| 超声萃取-三氟乙酰胺衍生化气相色谱-质谱法测定血液中阿片类毒品 |
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| 引用本文： | 王继芬,孙洪峰,孟品佳,王燕燕,张亮,国菲.超声萃取-三氟乙酰胺衍生化气相色谱-质谱法测定血液中阿片类毒品[J].理化检验(化学分册),2009(12). |
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| 作者姓名： | 王继芬 孙洪峰 孟品佳 王燕燕 张亮 国菲 |
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| 作者单位： | 中国人民公安大学刑事科学技术系;康师傅控股有限公司; |
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| 基金项目： | 北京市教育委员会重点实验室建设计划项目资助(JD100410669) |
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| 摘 要： | 在pH 9的磷酸盐缓冲介质中,人血试样(其中加乙基吗啡为内标)用氯仿、异丙醇及正庚烷(50+17+33)的混合溶剂进行超声萃取10 min,使其中吗啡、6-单乙酰吗啡和可待因进入有机相。离心分离,取上清液常温下吹干。残渣溶于乙酸乙酯中并用MBTFA试剂进行衍生化,取经衍生化的溶液1μL作气相色谱-质谱测定。人体血液中吗啡、6-单乙酰吗啡、可待因的检出限(3S/N)分别为1,2,3μg·L~(-1),标准曲线线性范围均为10～1 000μg·L~(-1)。1 mL血液中添加10,100,1 000 ng 3个浓度药物,平均相对回收率吗啡为82.4%～85.5%,6-单乙酰吗啡为95.7%～99.1%,可待因为97.5%～101.2%。相对标准偏差(RSD,n=5),RSD_(日内)为4.1%～11.2%,RSD_(日间)为4.9%～12.1%。
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| 关 键 词： | 气相色谱-质谱法 超声萃取 吗啡 6-单乙酰吗啡 可待因 人血 |
GC-MS Determination of Opiates in Human Blood with Ultrasonic Extraction and Trifluoro-acetylation Derivitization |
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| WANG Ji-fen,SUN Hong-feng,MENG Pin-jia,WANG Yan-yan,ZHANG Liang,GUO Fei. Dept. of Criminalistics , Technology,Chinese People s Public Security University,Beijing ,China,. Rang Shi Fu Stock Holding Co. Ltd.,Tianjin.GC-MS Determination of Opiates in Human Blood with Ultrasonic Extraction and Trifluoro-acetylation Derivitization[J].Physical Testing and Chemical Analysis Part B:Chemical Analgsis,2009(12). |
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| Authors: | WANG Ji-fen SUN Hong-feng MENG Pin-jia WANG Yan-yan ZHANG Liang GUO Fei Dept of Criminalistics Technology Chinese People s Public Security University Beijing China Rang Shi Fu Stock Holding Co Ltd Tianjin |
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| Institution: | WANG Ji-fen~1,SUN Hong-feng~2,MENG Pin-jia~1,WANG Yan-yan~1,ZHANG Liang~1,GUO Fei~11. Dept. of Criminalistics and Technology,Chinese People s Public Security University,Beijing 100038,China,2. Rang Shi Fu Stock Holding Co. Ltd.,Tianjin 300457 |
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| Abstract: | |
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| Keywords: | GC-MS Ultrasonic extraction Morphine 6-Monoacetylmorphine Codeine Human blood |
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