| 镉还原法测定血清NO稳定代谢产物NO3-/NO2- |
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| 引用本文： | 汪显阳,赵慧卿.镉还原法测定血清NO稳定代谢产物NO3-/NO2-[J].理化检验(化学分册),2005,41(6):417-419. |
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| 作者姓名： | 汪显阳 赵慧卿 |
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| 作者单位： | 安徽医科大学基础医学院,合肥,230032 |
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| 基金项目： | 安徽省教育厅自然科学基金资助课题 (No2003kj222);安徽医科大学优秀中青年骨干教师基金资助课题. |
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| 摘 要： | 建立了血清NO的间接测定方法。采用正交设计法L9(3^4)]观察不同还原剂及其用量，原时间和温度对NO3^-还原率的影响。最佳还原条件为400mg镀铜镉粒室温还原120min，还原率达97%以上。方法简便易行，不需昂贵设备，准确度较高，适合一般实验室用于各种生物医学实验中NO的间接测定。
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| 关 键 词： | 氮氧化物 硝酸盐 亚硝酸盐 镉 血清 |
| 文章编号： | 1001-4020(2005)06-0417-03 |
| 修稿时间： | 2004年2月6日 |
CADMIUM REDUCTION METHOD FOR DETERMINATION OF METABOLITES OF NITRATE AND NITRITE FROM NITROGEN OXIDES IN SERUM |
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| WANG Xian-yang,ZHAO Hui-qing.CADMIUM REDUCTION METHOD FOR DETERMINATION OF METABOLITES OF NITRATE AND NITRITE FROM NITROGEN OXIDES IN SERUM[J].Physical Testing and Chemical Analysis Part B:Chemical Analgsis,2005,41(6):417-419. |
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| Authors: | WANG Xian-yang ZHAO Hui-qing |
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| Abstract: | An indirect method for the determination of nitrogen oxides in serum via metabolites nitrate and nitrite was established in this paper. An orthogonal design L_9(3~4) was used to optimise the pasameters such as form and amount of the Cd-reductant, reduction time and reduction temperature. The maximum reduction sate 97% can be obtained under conditions that 400 mg copper-plating cadmium grains was used for reduction at soom temperature over 120 min. |
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| Keywords: | Nitric oxides Nitrate Nitrite Cadmium Serum |
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