| 高效液相色谱法测定柑橘皮中新橙皮苷 |
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| 引用本文： | 徐旭耀,苏欣,汪秋安.高效液相色谱法测定柑橘皮中新橙皮苷[J].理化检验(化学分册),2012(3):342-344. |
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| 作者姓名： | 徐旭耀 苏欣 汪秋安 |
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| 作者单位： | 湖南大学化学化工学院;湛江师范学院化学科学与技术学院 |
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| 摘 要： | 提出了高效液相色谱法测定柑橘皮中新橙皮苷含量的方法。样品用甲醇超声提取,定容至50mL。以SinoChrom ODS-BP色谱柱(250mm×4.6mm,10μm)为分离柱,以甲醇-水(36+64)溶液作为流动相,用紫外检测器在波长283nm处进行测定。新橙皮苷质量浓度在0.005 3～0.32g.L-1范围内与其峰面积呈线性关系,方法的检出限(3S/N)为0.6mg.L-1。方法的平均回收率为90.3%,相对标准偏差(n=6)为1.7%。
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| 关 键 词： | 高效液相色谱法 新橙皮苷 柑橘皮 |
HPLC Determination of Neohesperidin in Citrus Peels |
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| XU Xu-yao,SU Xin,WANG Qiu-an.HPLC Determination of Neohesperidin in Citrus Peels[J].Physical Testing and Chemical Analysis Part B:Chemical Analgsis,2012(3):342-344. |
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| Authors: | XU Xu-yao SU Xin WANG Qiu-an |
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| Institution: | 1(1.College of Chemistry and Chemical Engineering,Hunan University,Changsha 410082,China; 2.Dept.of Chemistry and Technology,Zhanjiang Normal College,Zhanjiang 524048,China) |
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| Abstract: | HPLC was applied to the determination of neohesperidin in citrus peels.The Sample was ultrasonically extracted with methanol,and made up to 50 mL.SinoChrom ODS-BP(250 mm×4.6 mm,10 μm) column was used as stationary phase,a mixture of methanol and water mixed in the ratio of 36 to 64(by volume) was used as mobile phase;UV-detection at 283 nm was used in the determination.Linear relationship between values of peak area and mass concentration of neohesperidin was obtained in the range of 0.005 3-0.32 g·L-1,with limit of detection(3S/N) of 0.6 mg·L-1.Values of average recovery and RSD(n=6) found were 90.3% and 1.7% respectively. |
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| Keywords: | HPLC Neohesperidin Citrus peels |
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