Au-assisted visible laser MALDI |
| |
Authors: | Lee Chuin Chen Kunihiko Mori Hirokazu Hori Kenzo Hiraoka |
| |
Institution: | 1. Clean Energy Research Center, University of Yamanashi, 4-3-11 Takeda, Kofu 400-8511, Japan;2. Interdisciplinary Graduate School of Medical and Engineering, University of Yamanashi, 4-3-11 Takeda, Kofu 400-8511, Japan |
| |
Abstract: | The excitation of UV-absorbing MALDI matrixes with visible laser (532 nm wavelength) and the desorption/ionization of biomolecules were performed by coating the analytes doped matrix with Au thin film (5–10 nm) using ion sputtering deposition. The Au film was first ablated with the laser of higher fluence, resulting in a crater/hole about the size of the laser beam spot on the target. After a few initial laser shots, analytes and matrix related ions were observed from the crater even at lower laser fluence. Electron microscopy inspection on the laser ablated region revealed the formation of nanoparticles with sizes ranging from <10 to 50 nm. Compared with the infra-red laser (1064 nm) excitation, the visible laser produced much higher abundance of matrix radical ions, and less heating effect as measured by the thermometer molecules. The results suggest the photo-excitation and photo-ionization of matrix molecules by the visible laser, possibly assisted by the gold nanoparticles and nanostructures left on the ablated crater. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|