The effect of methyl jasmonate and light irradiation treatments on the stilbenoid biosynthetic pathway in Vitis vinifera cell suspension cultures |
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| Authors: | Seyed Ali Andi Mansour Gholami Christopher M Ford |
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| Institution: | 1. Faculty of Agriculture, Department of Horticultural Sciences, Bu-Ali Sina University, Hamedan, Iran;2. Faculty of Science and Herbs, Department of Basic Science, Amol University of Special Modern Technologies, Amol, Iranandia@ut.ac.ir;4. School of Agriculture, Food and Wine, University of Adelaide, Adelaide, Australia |
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| Abstract: | Grape stilbenes are a well-known family of plant polyphenolics that have been confirmed to have many biological activities in relation to health benefits. In the present study, we investigated the effect of methyl jasmonate (MeJA) elicitor at four different concentrations (25, 50, 100 and 200 μM) in combination or not with high-level light irradiation (10,000 LUX) on a cell line obtained from the pulp of Vitis vinifera cv. Shahani. Our results showed that the stilbene synthesis pathway is inhibited by high-light conditions. A concentration of 50 μM MeJA was optimum for efficient production and high accumulation of total phenolics and total flavonoids as well as total stilbenoids. Furthermore, we showed that there is a significant negative correlation between the production of these metabolites and cell growth. These data provide valuable information for the future scale-up of cell cultures for the production of these very high value compounds in bioreactor system. |
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| Keywords: | Vitis vinifera cv Shahani cell suspension cultures MeJA elicitor light irradiation stilbenoids |
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