Modulation of antibiotic activity by the hydroalcoholic extract from leaves of Caryocar coriaceum WITTM |
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| Authors: | Luís J de Lacerda Neto A G B Ramos M R Kerntopf L J Quintans-Junior J R G S Almeida |
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| Institution: | 1. Departamento de Química Biológica, Universidade Regional do Cariri – URCA, Crato, Brasil;2. Unidade Acadêmica Ciências da Vida, Universidade Federal de Campina Grande, Cajazeiras, Brasil;3. Departamento de Fisiologia, Universidade Federal de Sergipe, S?o Cristov?o, Brasil;4. Departamento de Ciências Farmacêuticas, Universidade Federal do Vale do S?o Francisco, Petrolina, Brasil |
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| Abstract: | The aim of this study was to identify the presence of tannins, phenols and flavonoids on the hydroalcoholic extract of Caryocar coriaceum leaves (HECCL) and to determine the antioxidant and antibacterial activity of this extract. The extract was tested alone (1024–1 μg/mL) or associated (MIC/8) with several antibiotics in order to identify any antibacterial activity against multiresistant bacterial strains (Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa). The existence of tannins, total phenols (901.31 mg/g) and flavonoids (89.68 mg/g) was confirmed in the HECCL. The presence of rutin and quercetin were confirmed by Thin-layer chromatography (TLC). Using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method, the antioxidant activity of the extract (9 μg/mL) was determined. Moreover, the Minimum Inhibitory Concentration (MIC) value found for HECCL was 1024 μg/mL and the association between HECCL (MIC/8) with benzylpenicillin significantly changed its minimum inhibitory concentration from 2500 to 625 μg/mL against E. coli. |
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| Keywords: | Antibiotic antibiotic modifying activity synergism pequi |
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